FastQCFastQC Report
Thu 6 Dec 2018
HFJMNBGX9_n02_10x_ical_dec18_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJMNBGX9_n02_10x_ical_dec18_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71897194
Sequences flagged as poor quality0
Sequence length99
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2070170.28793474193165314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1322250.054.2175062
GCAGTGG1450450.049.226593
AGTGGTA1469000.048.5975385
AAGCAGT1508550.047.8674621
GTGGTAT1806600.039.56786
CAGTGGT1819750.039.432124
GGTATCA1844450.038.799258
GTATCAA1980100.036.030949
TGGTATC2000050.035.871557
ACGCAGA1627100.026.18030414-15
CAACGCA1715350.024.90276112-13
AGTACAT1752700.024.23467320-21
GCAGAGT1809000.023.71847316-17
ATCAACG1836350.023.47126210-11
TACATGG1826900.023.21141622-23
CATGGGG1058450.023.15617224-25
AGAGTAC1894650.022.647718-19
GTACATG1657450.021.34772522-23
TCAACGC1757300.020.29924612-13
TATCAAC1777000.020.16619710-11
Produced by FastQC (version 0.11.5)