FastQCFastQC Report
Thu 6 Dec 2018
HFJMNBGX9_n02_10x_ical_dec18_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJMNBGX9_n02_10x_ical_dec18_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81236663
Sequences flagged as poor quality0
Sequence length99
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2239690.2756994092679558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1511800.054.149392
GCAGTGG1668200.048.9809043
AGTGGTA1674400.048.859425
AAGCAGT1711250.048.224641
GTGGTAT2065950.039.6672366
CAGTGGT2074450.039.4906464
GGTATCA2099850.039.007578
GTATCAA2256750.036.182229
TGGTATC2291750.035.8509677
ACGCAGA1849950.026.27412414-15
CAACGCA1944900.025.04321912-13
AGTACAT1999400.024.29588920-21
GCAGAGT2067850.023.68182216-17
ATCAACG2079150.023.64226710-11
TACATGG2074200.023.3081922-23
CATGGGG1202550.023.192824-25
AGAGTAC2149400.022.81715218-19
GTACATG1890950.021.46852722-23
TCAACGC1989050.020.53392812-13
TATCAAC2022500.020.30029710-11
Produced by FastQC (version 0.11.5)