FastQCFastQC Report
Thu 6 Dec 2018
HFJMNBGX9_n02_10x_ical_dec18_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJMNBGX9_n02_10x_ical_dec18_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55646107
Sequences flagged as poor quality0
Sequence length99
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1576780.283358546537676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1016050.053.618832
GCAGTGG1119200.048.5358853
AGTGGTA1132200.048.1494035
AAGCAGT1154100.047.5918161
GTGGTAT1390000.039.283136
CAGTGGT1392450.039.199674
GGTATCA1413050.038.5938038
GTATCAA1523000.035.697589
TGGTATC1539350.035.5328837
ACGCAGA1244700.026.20793514-15
CAACGCA1320150.024.79330812-13
AGTACAT1351550.024.05178520-21
GCAGAGT1392900.023.6093116-17
ATCAACG1415500.023.30278810-11
TACATGG1401100.023.13105222-23
CATGGGG802050.022.92862124-25
AGAGTAC1446350.022.69459218-19
GTACATG1272650.021.1793922-23
TCAACGC1353050.020.16071112-13
TATCAAC1366800.020.00259210-11
Produced by FastQC (version 0.11.5)