FastQCFastQC Report
Thu 6 Dec 2018
HFJMNBGX9_n02_10x_ical_dec18_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJMNBGX9_n02_10x_ical_dec18_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66677438
Sequences flagged as poor quality0
Sequence length99
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1853660.278004082880329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1237000.054.781492
GCAGTGG1361250.049.582253
AGTGGTA1368000.049.4555445
AAGCAGT1410550.048.427111
GTGGTAT1685900.040.1606526
CAGTGGT1694400.040.084684
GGTATCA1723350.039.1994368
GTATCAA1842850.036.6246539
TGGTATC1868800.036.3674137
ACGCAGA1523550.026.42763514-15
CAACGCA1591500.025.36088212-13
AGTACAT1635300.024.53660820-21
GCAGAGT1686750.024.01086816-17
ATCAACG1712500.023.73614710-11
TACATGG1707350.023.39840722-23
CATGGGG981000.023.33218224-25
AGAGTAC1764450.022.94404818-19
GTACATG1547700.021.69706522-23
TCAACGC1632350.020.70512812-13
TATCAAC1656050.020.50163810-11
Produced by FastQC (version 0.11.5)