FastQCFastQC Report
Thu 6 Dec 2018
HFJMNBGX9_n02_10x_ctr_dec18_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJMNBGX9_n02_10x_ctr_dec18_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74569470
Sequences flagged as poor quality0
Sequence length99
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2791140.3743006353672622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1758000.059.509322
AAGCAGT1877150.055.9751321
GCAGTGG2021300.051.6189583
AGTGGTA2073050.050.4622085
CAGTGGT2306900.045.3947874
GTGGTAT2463550.042.5031136
GGTATCA2569000.040.654288
TGGTATC2682850.039.0485427
GTATCAA2731850.038.1881879
ACGCAGA2327050.025.95219814-15
CAACGCA2410750.025.08057212-13
GCAGAGT2502300.024.27590416-17
ATCAACG2539150.023.90218710-11
AGTACAT2535800.023.86780420-21
CATGGGG1436150.023.18938824-25
AGAGTAC2696350.022.57484818-19
TACATGG2674350.022.53677422-23
TCAACGC2428700.021.47455612-13
CGCAGAG2467750.021.32739416-17
AACGCAG2478300.021.17383214-15
Produced by FastQC (version 0.11.5)