FastQCFastQC Report
Thu 6 Dec 2018
HFJMNBGX9_n02_10x_ctr_dec18_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJMNBGX9_n02_10x_ctr_dec18_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83904326
Sequences flagged as poor quality0
Sequence length99
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3206720.3821876836243223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1983900.059.6984522
AAGCAGT2124200.056.044521
GCAGTGG2283750.051.7033353
AGTGGTA2360950.050.152135
CAGTGGT2616150.045.2720074
GTGGTAT2791350.042.436186
GGTATCA2920600.040.5114068
TGGTATC3063850.038.7158627
GTATCAA3095900.038.160289
ACGCAGA2639300.025.92022914-15
CAACGCA2736250.024.99725312-13
GCAGAGT2830050.024.30882616-17
ATCAACG2883650.023.86887610-11
AGTACAT2876100.023.86752320-21
CATGGGG1632750.023.08276724-25
AGAGTAC3056500.022.58192618-19
TACATGG3034250.022.52092422-23
TCAACGC2748400.021.5044412-13
CGCAGAG2802750.021.28358716-17
AACGCAG2804150.021.18674714-15
Produced by FastQC (version 0.11.5)