FastQCFastQC Report
Thu 6 Dec 2018
HFJMNBGX9_n02_10x_ctr_dec18_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJMNBGX9_n02_10x_ctr_dec18_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54918674
Sequences flagged as poor quality0
Sequence length99
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2124190.3867882898993519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1297150.059.815522
AAGCAGT1394950.056.0222741
GCAGTGG1501750.051.5945973
AGTGGTA1552650.049.9951325
CAGTGGT1717000.045.3036354
GTGGTAT1831800.042.4121676
GGTATCA1914000.040.5234878
TGGTATC2008500.038.741817
GTATCAA2025900.038.2299929
ACGCAGA1729400.025.9117814-15
CAACGCA1789100.025.08109912-13
GCAGAGT1853850.024.32125916-17
AGTACAT1880050.023.9559320-21
ATCAACG1885650.023.9119510-11
CATGGGG1064350.023.20677624-25
AGAGTAC1998500.022.65067718-19
TACATGG1986200.022.5637822-23
TCAACGC1798750.021.54562412-13
CGCAGAG1833850.021.25310716-17
AACGCAG1832500.021.20575714-15
Produced by FastQC (version 0.11.5)