FastQCFastQC Report
Thu 6 Dec 2018
HFJMNBGX9_n02_10x_ctr_dec18_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJMNBGX9_n02_10x_ctr_dec18_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65107709
Sequences flagged as poor quality0
Sequence length99
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2595630.3986670764286914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1515400.059.5165562
AAGCAGT1623150.055.875091
GCAGTGG1748650.051.5486683
AGTGGTA1803150.050.0805665
CAGTGGT1998300.045.2169954
GTGGTAT2135050.042.3565376
GGTATCA2232850.040.377248
TGGTATC2338200.038.699157
GTATCAA2359500.038.1250889
ACGCAGA2004100.026.02272614-15
CAACGCA2069050.025.21066512-13
GCAGAGT2158400.024.34204516-17
ATCAACG2185800.024.02715510-11
AGTACAT2191600.023.95678720-21
CATGGGG1259050.023.17137524-25
AGAGTAC2338350.022.56321118-19
TACATGG2321800.022.50657822-23
TCAACGC2087600.021.55284912-13
CGCAGAG2130850.021.32423816-17
AACGCAG2131000.021.23146414-15
Produced by FastQC (version 0.11.5)