FastQCFastQC Report
Thu 6 Dec 2018
HFJMNBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJMNBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31002316
Sequences flagged as poor quality0
Sequence length27
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGC435530.14048305294352847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGC50500.018.85145421
GGGACCT43050.017.9286611
GGGAATG64500.017.8758431
CGTGAGC50900.017.7773361
GCGCGAT47100.017.5527211
GACTAAC61450.017.4572351
TAGTTGG86950.017.3769571
ATTGGAC82150.017.3255081
ACGAGGA45750.017.0552061
TGCGGGT49450.017.0174961
CACCGAA49500.016.9850758
GTCGACT39400.016.7835758
CCGGTAG62600.016.7148691
GGCTCGA46500.016.7120041
TTAGGCA80450.016.7072891
CGATGGC78100.016.6421971
ATCGAGT71900.016.594081
GAGGCAC33300.016.5821914
CTCGAGG52700.016.5490511
ACGAGCC56700.016.5361161
Produced by FastQC (version 0.11.5)