FastQCFastQC Report
Thu 6 Dec 2018
HFJMNBGX9_n01_10x_ical_dec18_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJMNBGX9_n01_10x_ical_dec18_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71897194
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGGC190900.018.5433461
CTGCGGA179200.018.3593671
GATGAAA269750.018.2421531
CTAAGAC224300.018.193191
CGTGAGC167100.018.1617071
ACGAGCC230250.018.156321
CGCTGGA207050.018.0656661
ATAACGC207600.017.9773371
ACTGAAC180600.017.8390981
TATGCCC235600.017.7752291
ACGGAAG204100.017.7553943
GGGAATG176000.017.7385251
TAGTTGG280600.017.7351261
GTAACTG284300.017.670531
GGGACCT154600.017.6127971
ACGCCGA236200.017.583361
ACGGAGA250350.017.4871731
CGTTGGG285400.017.4736441
AAACCTG229650.017.4081151
GGGATAC157500.017.4061379
Produced by FastQC (version 0.11.5)