FastQCFastQC Report
Thu 6 Dec 2018
HFJMNBGX9_n01_10x_ical_dec18_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJMNBGX9_n01_10x_ical_dec18_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81236663
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGGC219300.018.6555541
CTAAGAC246400.018.2530381
CGCTGGA241500.018.2494451
ACGAGCC278350.018.2214811
CGTGAGC192850.018.2085111
CTGCGGA200150.018.1477491
GATGAAA323250.018.1333941
ACTGAAC209000.017.997291
GGGAATG196000.017.7765771
ATAACGC239250.017.7583121
TAGTTGG319200.017.7285441
ACGGAAG225400.017.6564753
GTAACTG327700.017.582731
TATGCCC260550.017.555961
CGTTGGG325500.017.5209061
ACGCCGA259950.017.4874131
AAACCTG255450.017.4789431
ACGAGGA178750.017.44771
TGGACGC203200.017.3895841
GGGACCT175150.017.3866391
Produced by FastQC (version 0.11.5)