FastQCFastQC Report
Thu 6 Dec 2018
HFJMNBGX9_n01_10x_ical_dec18_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJMNBGX9_n01_10x_ical_dec18_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55646107
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGGC149400.018.668511
CTGCGGA138500.018.3938711
ACGAGCC181550.018.318251
CTAAGAC167750.018.1913621
GATGAAA216450.018.1882111
CGTGAGC132900.018.1575491
CGCTGGA159050.018.1168441
ATAACGC161850.017.9137231
GGGAATG133500.017.887161
TAGTTGG214600.017.8214231
ACGCCGA178650.017.7985651
ACGGAAG159850.017.6842233
ACTGAAC142600.017.5704571
TGGACGC144650.017.5537531
GACTAAC136300.017.4965921
TATGCCC174300.017.489681
CGTTGGG224100.017.4782981
GGGACCT123450.017.4719541
AAACCTG178000.017.4152011
GTAACTG211900.017.3943561
Produced by FastQC (version 0.11.5)