FastQCFastQC Report
Thu 6 Dec 2018
HFJMNBGX9_n01_10x_ical_dec18_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJMNBGX9_n01_10x_ical_dec18_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66677438
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGGC174450.018.6544421
ACGAGCC223600.018.2546881
CTAAGAC207000.018.2220671
GATGAAA261250.018.1321031
CGTGAGC157100.018.0542761
CTGCGGA166300.018.02161
GGGAATG157650.018.0046121
CGCTGGA200800.017.8486041
ACTGAAC171700.017.7911951
ACGCCGA214300.017.7385481
GGGACCT143900.017.7181971
ACGGAAG192250.017.6644233
ATAACGC192500.017.652631
GTAACTG262050.017.5598131
TAGTTGG255900.017.5222241
AAACCTG211850.017.4975761
TGGACGC168750.017.486151
CGTTGGG264000.017.4619311
ACGAGGA144150.017.4543551
ATAGACC184200.017.3819891
Produced by FastQC (version 0.11.5)