FastQCFastQC Report
Thu 6 Dec 2018
HFJMNBGX9_n01_10x_ctr_dec18_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJMNBGX9_n01_10x_ctr_dec18_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74569470
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGGA282250.018.8888841
ATTGGAC422050.018.670761
TGGACGC245650.018.6595231
TGACGGC226650.018.6160411
GGATTAC323050.018.6096761
AAATGCC374550.018.2293111
TGCTACC284050.018.133321
GGGAATG272400.018.0954381
GACTAAC259550.018.0648161
CACTGCC241650.018.031748
CAATGGA191000.017.9649098
CACCGAA215400.017.923568
CGGCCGA168450.017.9015149
ATCGAGT290950.017.851261
CCGTGGA195150.017.843461
CTCGAGG223650.017.8140031
ATAACGC228900.017.8137231
GCGCGAT197800.017.8061431
ACACTGA313700.017.805271
GGGACCT170800.017.7128521
Produced by FastQC (version 0.11.5)