FastQCFastQC Report
Thu 6 Dec 2018
HFJMNBGX9_n01_10x_ctr_dec18_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJMNBGX9_n01_10x_ctr_dec18_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83904326
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGGA318700.018.9786451
TGGACGC281050.018.7748851
TGACGGC250600.018.7389621
ATTGGAC476500.018.6591321
GGATTAC367850.018.6466261
GGGAATG310300.018.2369081
AAATGCC430100.018.1891171
CCGTGGA214800.018.1858441
GACTAAC296700.018.099561
TGCTACC319500.018.0601621
CGGCCGA196250.018.008689
CACTGCC259400.018.0041148
CACCGAA240450.018.0038538
GCGCGAT217300.017.9476261
ATCGAGT325200.017.8986031
ATAACGC261250.017.8584941
CCGAACG208850.017.8020049
ACCCACT330700.017.7438141
CAATGGA218500.017.7269558
TGAGCAT306100.017.7015461
Produced by FastQC (version 0.11.5)