FastQCFastQC Report
Thu 6 Dec 2018
HFJMNBGX9_n01_10x_ctr_dec18_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJMNBGX9_n01_10x_ctr_dec18_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54918674
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGGA199850.019.0161861
TGGACGC183100.018.7140461
TGACGGC157250.018.711841
ATTGGAC311050.018.7100661
GGATTAC238500.018.651191
AAATGCC278800.018.324371
GGGAATG209100.018.2804261
CACTGCC176550.018.1801838
GACTAAC188500.018.1612281
CACCGAA160500.018.1010888
TGCTACC207550.018.0880721
CGGCCGA129800.018.078999
CCGTGGA140500.018.0650561
GCGCGAT143000.018.0429731
ACACTGA235700.017.9282281
ATAACGC165700.017.9223881
CAATGGA141400.017.8655648
CCGAACG136150.017.8527329
GTCGACT152750.017.816588
CGAACGC137050.017.76610810
Produced by FastQC (version 0.11.5)