FastQCFastQC Report
Thu 6 Dec 2018
HFJMNBGX9_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJMNBGX9_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584959898
Sequences flagged as poor quality0
Sequence length99
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21159330.3617227449666986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG12081250.057.467692
AAGCAGT13375050.052.271631
GCAGTGG13779400.050.2573853
AGTGGTA14104350.049.184515
CAGTGGT16342250.042.536154
GTGGTAT16937350.041.0063256
GGTATCA17475800.039.6917428
GTATCAA18575300.037.2786839
TGGTATC18682650.037.2421847
ACGCAGA15824500.025.69461414-15
CAACGCA16285150.024.99186512-13
AGTACAT16904600.024.09525920-21
GCAGAGT17023150.024.04455816-17
ATCAACG17229000.023.77798310-11
CATGGGG9877800.023.05277424-25
TACATGG17705000.022.90514422-23
AGAGTAC18074800.022.7109218-19
GTACATG16440700.021.0757922-23
CGCAGAG16847800.020.75358216-17
TCAACGC16727800.020.72618912-13
Produced by FastQC (version 0.11.5)