FastQCFastQC Report
Thu 6 Dec 2018
HFJMNBGX9_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJMNBGX9_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584959898
Sequences flagged as poor quality0
Sequence length6
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCATG8390432614.343603089181336No Hit
GCAGTA8123666313.887561058074446No Hit
GAAGGA7456947112.74779198624655No Hit
TTCCCG7189719412.290961183120283No Hit
CAGTAC6667743811.398634030806672No Hit
TTTCAT6510770911.130285891837325No Hit
AGTAGT556461079.51280714973046No Hit
ACGTCC549186749.388451103702838No Hit
GGGGGG130933572.238334122521336No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)