FastQCFastQC Report
Thu 6 Dec 2018
HFJMNBGX9_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJMNBGX9_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584959898
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGGA1805000.018.6111161
TGACGGC1658050.018.2390251
TGGACGC1664650.018.0613331
GGGAATG1755400.017.989881
CGTGAGC1463450.017.8700891
GACTAAC1674700.017.8419281
GGATTAC1976550.017.7568231
ATAACGC1717250.017.7319831
GATGAAA1950950.017.7200811
GCGCGAT1309900.017.5744111
GGGACCT1268300.017.5578561
TAGTTGG2413550.017.5347081
ATCGAGT2075250.017.4852711
ACGAGGA1245900.017.3567121
TGCTACC1819050.017.3165131
AAATGCC2572000.017.3035341
ACGGAGA1997050.017.299461
CACCGAA1280900.017.2824848
ACGAGCC1693350.017.185151
CGATGGC2189500.017.1389771
Produced by FastQC (version 0.11.5)