FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86978032
Sequences flagged as poor quality0
Sequence length25
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT42125584.843243636508125No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG9660031.1106287159957815No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG6558140.7539995846307491No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4381880.5037915780849123No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT4035920.464016017285836No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT3101850.3566245325026439No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA3007090.34572982750403No Hit
TTTTTTTTTTTTTTTTTTTTTTATT1781260.20479424045832634No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT1680250.1931809632114923No Hit
GCGGGGGGGGGGGGGGGGGGGGGGG1395800.160477303050499No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1352750.15552777740475893No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA1339850.15404464428443265No Hit
TTTTTTATTTTTTTTTTTTTTTTTT1183580.1360780386477358No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG1060080.12187905102290655No Hit
TTTTATTTTTTTTTTTTTTTTTTTT1006240.11568898224783933No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC930450.10697528773702307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTGG16450.018.4730410
GGGGCAA20500.017.87979111
GGGCAAA22850.017.4514812
GCAAGTG14750.017.3825119
GGGGGCA20650.017.29107910
AAGTGGG21700.017.06608411
CAGTGGG15400.016.89502711
GGCAAAA30550.016.84298113
CAAAAAG25600.016.42771315
AAGGTGG10150.016.35490819
TAAAGGG4550.016.27404814
AGTGGGG32150.016.24242612
GTGCAAG15250.016.1958337
CCAGTGG17100.016.10483210
GTGAAAA11500.016.0992612
AAGTCGG5200.015.8871111
AAGTTTG24100.015.744143519
GCAAAAA26900.015.52790114
AAAGGTG10550.015.47053818
AAACGGG5400.015.46399117
Produced by FastQC (version 0.11.5)