FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n02_S_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n02_S_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12293462
Sequences flagged as poor quality0
Sequence length25
%GC7

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT269281021.904407399640558No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4146623.373028687931846No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG3451792.8078258183089515No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA3031502.4659449063250043No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT2167321.7629858863190857No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA1340831.0906854391382999No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT1284781.0450920985479923No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1166000.9484716347600051No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT993060.8077952329457723No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC992710.8075105287672423No Hit
TTTTTTTTTTTTTTTTTTTTTTATT837230.6810367982591071No Hit
TTTTTTTTTTTTTTTTTTTTTTACA650630.529248799077103No Hit
TTTTTTATTTTTTTTTTTTTTTTTT611600.4975002159684554No Hit
TTTTTTTTTTTTTTTTTTTTTTACC579630.4714945228610134No Hit
TTTTTATTTTTTTTTTTTTTTTTTT547960.4457328619065972No Hit
TTTTATTTTTTTTTTTTTTTTTTTT513390.4176122234729322No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG482820.3927453470796103No Hit
TTATTTTTTTTTTTTTTTTTTTTTT478090.38889777346690463No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC461030.3750204783648414No Hit
TTTTTTTTATTTTTTTTTTTTTTTT448330.36468978388675216No Hit
TTTATTTTTTTTTTTTTTTTTTTTT415310.33782997824372013No Hit
TTTTTTTATTTTTTTTTTTTTTTTT380640.30962799575904654No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC376990.30665893789723353No Hit
TTTTTTTTTATTTTTTTTTTTTTTT342880.27891248209820796No Hit
TTTTTTTTTTATTTTTTTTTTTTTT342230.2783837457666522No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA329440.26797984164265526No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA320340.2605775330008748No Hit
TTTTTTTTTTTATTTTTTTTTTTTT303010.24648060896108842No Hit
TTTTTTTTTTTTTTTTTTTTTAATT297520.24201482056071755No Hit
TTTTTTTTTTTTATTTTTTTTTTTT296820.2414454122036575No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA284170.23115538975107255No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC276820.22517660200194214No Hit
TTTTTTTTTTTTTTATTTTTTTTTT257430.20940399051137915No Hit
TTTTTTTTTTTTTATTTTTTTTTTT254450.20697993779132356No Hit
TTTTTTTTTTTTTTTATTTTTTTTT244240.19867471018334787No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT241370.1963401359194017No Hit
TTTTTTTTTTTTTTTTTTTTTACCA235450.19152456809969398No Hit
TTTTTTTTTTTTTTTTATTTTTTTT224630.18272314178056595No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG217720.1771022678558733No Hit
TTTTTTTTTTTTTTTTTTTTTATTT205420.16709694958181837No Hit
TTTTTTTTTTTTTTTTTTTTTTAAG188120.15302442875733457No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT181580.14770452782137367No Hit
TTTTTTTTTTTTTTTTTATTTTTTT175390.14266933106394278No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT173010.14073334264993864No Hit
TTTTTTTTTTTTTTTTTTTTTTATA169390.13778868800342817No Hit
TTTTTTTTTTTTTTTTTTTTTACCC166490.13542971052417943No Hit
TTTTTTTTTTTTTTTTTTATTTTTT160450.1305165298432614No Hit
TATTTTTTTTTTTTTTTTTTTTTTT159700.12990644946069707No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT156320.12715702053660718No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT153000.12445639804312242No Hit
TTTTTTTTTTTTTTTTTTTTTACAA152450.12400900576257526No Hit
TTTTTTTTTTTTTTTTTTTTTTACT151560.12328504370859893No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT146570.11922597556327094No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA134080.10906610359229972No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC132320.10763444829454875No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT129780.10556830939893092No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA124030.10089102646593776No Hit
TTTTTTTTTTTTTTTTTTTTTAACA122990.10004504833544856No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA307.656979E-419.0256921
GCCCCTA654.802132E-1019.0256921
CCCGAAA307.7142357E-419.0046273
CCGAAAC250.006035414518.9994474
GGTAAAG950.018.99836312
GTAAGTG359.95954E-518.9968199
AAACTGT307.742787E-418.9941918
TTACAAG250.006047765718.99272319
AACGGTT502.1850974E-718.99272319
AAATTGC307.746813E-418.99272219
CCTAAAC3750.018.4927964
CTAAACC4050.018.0604695
GTAAAAG1550.017.77244613
CCTAACC651.00499165E-817.537954
TCCCCTA3650.017.4619371
AAACGGT555.936836E-717.26744718
ATTTACG555.941638E-717.26611119
GAAGGTG504.5703437E-617.09887511
GTAAAGG1000.017.09831813
CCTCAAC951.8189894E-1216.9995044
Produced by FastQC (version 0.11.5)