FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n02_S_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n02_S_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11527405
Sequences flagged as poor quality0
Sequence length25
%GC9

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT252923821.941087347933035No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA3909183.3912055662137313No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG3396092.946101052231617No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2840532.4641539010731384No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT2101261.8228387048082373No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT1310421.1367866401848465No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA1247031.081795946268913No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1124930.9758744487592828No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT949000.8232555375646122No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC926670.803884308740779No Hit
TTTTTTTTTTTTTTTTTTTTTTATT825680.7162756925778179No Hit
TTTTTTTTTTTTTTTTTTTTTTACA616090.5344568009886006No Hit
TTTTTTTTTTTTTTTTTTTTTTACC531710.46125732547785037No Hit
TTTTTTATTTTTTTTTTTTTTTTTT513880.4457898373484752No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG479230.41573103400114775No Hit
TTTTTATTTTTTTTTTTTTTTTTTT452010.3921177402893366No Hit
TTTTATTTTTTTTTTTTTTTTTTTT437390.3794349205220082No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC430740.3736660592735312No Hit
TTATTTTTTTTTTTTTTTTTTTTTT402790.3494194920712858No Hit
TTTTTTTTATTTTTTTTTTTTTTTT369950.3209308599810625No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC344480.29883568765042956No Hit
TTTATTTTTTTTTTTTTTTTTTTTT337750.2929974265673844No Hit
TTTTTTTATTTTTTTTTTTTTTTTT323550.2806789559315388No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA309580.2685600098200766No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA289490.25113197636415135No Hit
TTTTTTTTTATTTTTTTTTTTTTTT287360.2492842057687745No Hit
TTTTTTTTTTATTTTTTTTTTTTTT282320.2449120161909814No Hit
TTTTTTTTTTTTTTTTTTTTTAATT279880.242795321236653No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA260760.2262087607748665No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC258370.2241354407171432No Hit
TTTTTTTTTTTATTTTTTTTTTTTT248430.21551251127205126No Hit
TTTTTTTTTTTTATTTTTTTTTTTT245270.21277121780660957No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT240850.20893687694671959No Hit
TTTTTTTTTTTTTTTTTTTTTACCA218370.18943552343307102No Hit
TTTTTTTTTTTTTTATTTTTTTTTT216140.187501003044484No Hit
TTTTTTTTTTTTTATTTTTTTTTTT207430.17994509605587727No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG207390.1799103961385932No Hit
TTTTTTTTTTTTTTTATTTTTTTTT206790.17938989737933214No Hit
TTTTTTTTTTTTTTTTTTTTTATTT199640.17318728716480422No Hit
TTTTTTTTTTTTTTTTATTTTTTTT187150.1623522379928527No Hit
TTTTTTTTTTTTTTTTTTTTTTAAG183200.15892562116105055No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT181720.15764172422153988No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT172720.14983424283262364No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT168860.14648570081471068No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT162530.14099443890450628No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT161840.14039586533135603No Hit
TTTTTTTTTTTTTTTTTTTTTTATA151320.13126978708564505No Hit
TTTTTTTTTTTTTTTTTTTTTACCC148300.12864994333069757No Hit
TTTTTTTTTTTTTTTTTTTTTTACT147450.12791257008841106No Hit
TTTTTTTTTTTTTTTTTATTTTTTT142590.12369653013839628No Hit
TTTTTTTTTTTTTTTTTTTTTACAA138660.1202872632652362No Hit
TATTTTTTTTTTTTTTTTTTTTTTT137250.11906409118097264No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT136030.11800574370380845No Hit
TTTTTTTTTTTTTTTTTTATTTTTT132620.1150475757553413No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA123110.10679767042105312No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC117380.1018269072701098No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA401.271057E-519.026421
TCGCCAC307.654965E-419.026421
TCCGCTA359.841169E-519.026421
GCCCCTA654.802132E-1019.026421
CCGCTAA307.6867884E-419.0146882
CGCCTAA401.2778362E-519.0146872
GCCTAAA552.833076E-819.006353
CGCTAAA451.6649665E-619.006353
CCTCAAC706.366463E-1119.0002484
CTAACAC250.006036643418.9987645
CGAAACC250.006036643418.9987645
GTAAAAG1950.018.99760813
TCTGAAA359.956618E-518.99752811
CTGTAAA307.733605E-418.9975266
ATTAGGG307.7354105E-418.99686810
GTCTGAA307.7354105E-418.99686810
TACCCAG401.2898005E-518.99414819
CCCTAAA5300.018.6477413
CCTAAAC5550.018.4867274
TCCCCTA5600.018.1770271
Produced by FastQC (version 0.11.5)