FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n02_S_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n02_S_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2576710
Sequences flagged as poor quality0
Sequence length25
%GC8

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT54477621.142309379014325No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA881463.4208739050960335No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG783513.04073799535066No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA628592.439506192004533No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT497561.9309895176407121No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT307101.1918298916059626No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA274891.0668255255733086No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC265081.0287537208300506No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT229330.8900109053793404No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC212140.8232979264255582No Hit
TTTTTTTTTTTTTTTTTTTTTTATT190510.739353671930485No Hit
TTTTTTTTTTTTTTTTTTTTTTACA136270.5288526842368757No Hit
TTTTTTTTTTTTTTTTTTTTTTACC118210.4587633066973001No Hit
TTTTTTATTTTTTTTTTTTTTTTTT117480.4559302366195652No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG116300.45135075348021314No Hit
TTTTTATTTTTTTTTTTTTTTTTTT104600.4059440138781624No Hit
TTTTATTTTTTTTTTTTTTTTTTTT102820.39903597998998724No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC98580.38258088803163726No Hit
TTATTTTTTTTTTTTTTTTTTTTTT94720.36760054488087524No Hit
TTTTTTTTATTTTTTTTTTTTTTTT85240.3308094430494701No Hit
TTTATTTTTTTTTTTTTTTTTTTTT80630.3129184114626792No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC78080.30302207078018095No Hit
TTTTTTTATTTTTTTTTTTTTTTTT74450.2889343387498011No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA71760.2784946695592442No Hit
TTTTTTTTTTTTTTTTTTTTTAATT66670.2587407973733947No Hit
TTTTTTTTTATTTTTTTTTTTTTTT66390.2576541403572773No Hit
TTTTTTTTTTATTTTTTTTTTTTTT66390.2576541403572773No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA60870.2362314734681047No Hit
TTTTTTTTTTTATTTTTTTTTTTTT60710.23561052660175186No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT59050.22916820286334122No Hit
TTTTTTTTTTTTATTTTTTTTTTTT58210.22590823181498887No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC57110.2216392221088132No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA52900.20530055768790434No Hit
TTTTTTTTTTTTTTATTTTTTTTTT50610.19641325566322945No Hit
TTTTTTTTTTTTTATTTTTTTTTTT48590.188573801475525No Hit
TTTTTTTTTTTTTTTATTTTTTTTT47570.1846152652025257No Hit
TTTTTTTTTTTTTTTTTTTTTACCA45690.17731913952287995No Hit
TTTTTTTTTTTTTTTTATTTTTTTT45120.175107016311498No Hit
TTTTTTTTTTTTTTTTTTTTTATTT44990.1746024969825863No Hit
TTTTTTTTTTTTTTTTTTTTTTAAG43750.1697901587683519No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG43420.16850945585649918No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT40560.15741003062044234No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT36650.14223564157394508No Hit
TTTTTTTTTTTTTTTTTTTTTTACT36070.13998470918341605No Hit
TTTTTTTTTTTTTTTTTATTTTTTT34660.13451261492368174No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT34250.13292143857865263No Hit
TTTTTTTTTTTTTTTTTTTTTTATA32750.12710006170659485No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT32320.12543126700327162No Hit
TTTTTTTTTTTTTTTTTTTTTACCC31190.12104582975965476No Hit
TATTTTTTTTTTTTTTTTTTTTTTT30950.12011440946012551No Hit
TTTTTTTTTTTTTTTTTTATTTTTT30790.11949346259377266No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT30580.11867846983168459No Hit
TTTTTTTTTTTTTTTTTTTTTACAA27610.10715214362501017No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA25980.10082624742404074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAAAG451.6712002E-618.99771113
TGTGAAA250.00603733218.9977111
TCCCATT250.0060400418.99623719
GGGTTAG307.7349175E-418.99623519
CTAAACC1100.018.1338255
CCTAAAC1050.018.0934094
TTTACAG751.72804E-1017.72981819
CCCTAAA1250.017.4850183
CCCCTAA1100.017.2840632
TCCCCTA1050.017.2164731
TTGTAAG402.7661797E-416.62170619
CCCCCAT402.7661797E-416.62170617
TTTTAAG8500.016.53789919
TCCCCAA1050.016.3103431
CCCATCC350.002173596316.28248819
CCCCGCT350.002173596316.28248817
CCCCATC350.002173878316.28217118
CCCCCTA651.9194522E-716.07342518
AAAGGTT551.1299662E-515.54388116
TGAAAAA551.13017395E-515.54358113
Produced by FastQC (version 0.11.5)