FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n02_S_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n02_S_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19389122
Sequences flagged as poor quality0
Sequence length25
%GC8

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT427582922.052721108258538No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA6924593.5713788380928237No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG5639332.908501994056255No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA4985382.57122524681623No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT3716041.9165591923141232No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT2181281.1250019469679957No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA2167891.1180960128055308No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1994781.0288139916804897No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT1716850.8854707294120899No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC1636890.8442311106196557No Hit
TTTTTTTTTTTTTTTTTTTTTTATT1467570.7569037937870523No Hit
TTTTTTTTTTTTTTTTTTTTTTACA1068370.5510151516917579No Hit
TTTTTTTTTTTTTTTTTTTTTTACC945070.48742279304859704No Hit
TTTTTTATTTTTTTTTTTTTTTTTT889330.45867471461575204No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG804350.41484601520378284No Hit
TTTTTATTTTTTTTTTTTTTTTTTT799920.41256122892000985No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC770540.3974084025052811No Hit
TTTTATTTTTTTTTTTTTTTTTTTT770010.39713505335620664No Hit
TTATTTTTTTTTTTTTTTTTTTTTT698180.3600885073599516No Hit
TTTTTTTTATTTTTTTTTTTTTTTT643760.3320212230342354No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC613610.3164712667236814No Hit
TTTATTTTTTTTTTTTTTTTTTTTT606080.3125876457943789No Hit
TTTTTTTATTTTTTTTTTTTTTTTT557760.2876664554485757No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA547390.28231809568272354No Hit
TTTTTTTTTTATTTTTTTTTTTTTT511920.26402433281919624No Hit
TTTTTTTTTATTTTTTTTTTTTTTT510870.2634827920521621No Hit
TTTTTTTTTTTTTTTTTTTTTAATT508180.2620954161823315No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA500270.2580158090706738No Hit
TTTTTTTTTTTATTTTTTTTTTTTT452300.23327513231388197No Hit
TTTTTTTTTTTTATTTTTTTTTTTT448360.231243065054725No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC444810.22941214150903788No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA437280.22552852057973535No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT434580.22413598717879024No Hit
TTTTTTTTTTTTTTATTTTTTTTTT395160.20380499952499137No Hit
TTTTTTTTTTTTTATTTTTTTTTTT380300.19614090828867858No Hit
TTTTTTTTTTTTTTTTTTTTTACCA373500.1926337871307427No Hit
TTTTTTTTTTTTTTTATTTTTTTTT364790.188141577529916No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG352730.18192159500569444No Hit
TTTTTTTTTTTTTTTTTTTTTATTT350460.18075083544267762No Hit
TTTTTTTTTTTTTTTTATTTTTTTT348650.17981732231093292No Hit
TTTTTTTTTTTTTTTTTTTTTTAAG306790.1582278970651688No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT302690.15611330930817807No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT280270.1445501245492189No Hit
TTTTTTTTTTTTTTTTTTTTTTACT273150.14087796239561542No Hit
TTTTTTTTTTTTTTTTTTTTTTATA270160.1393358605923466No Hit
TTTTTTTTTTTTTTTTTATTTTTTT268760.13861380623630096No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT267070.1377421834779316No Hit
TTTTTTTTTTTTTTTTTTTTTACCC258490.13331702178159485No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT258380.13326028893933414No Hit
TTTTTTTTTTTTTTTTTTATTTTTT243000.1253280060850615No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT242750.12519906780719622No Hit
TTTTTTTTTTTTTTTTTTTTTACAA239850.12370338378395886No Hit
TATTTTTTTTTTTTTTTTTTTTTTT227700.11743698347970578No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA216960.11189779506261294No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT212430.1095614334676939No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC210220.10842161909136473No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA195970.10107213725304323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCTA451.6496069E-619.0249081
TCGCCTA307.659323E-419.0249061
TCCCCGA401.2720029E-519.0249061
CGCCTAA307.689581E-419.0137562
CCGCTAA250.0060093519.0137542
GCCTAAA307.7128434E-419.0052193
CTGGTGA250.006037452318.9984045
GTAAAGG2050.018.99791313
GTAAAGT603.7325663E-918.99791113
TAAAGTG654.8930815E-1018.99786414
TAAGTCG359.957286E-518.99747310
TAGTAGG401.2879298E-518.99747310
GAGTTTC250.006039161718.9974710
ATAGGTA250.006040781318.996599
ACCCGTG502.1823507E-718.9951218
AACTGTT250.006046273318.993619
CACGTGT250.006046273318.993619
ACCGTGT359.972869E-518.993619
AACGGTT451.6758131E-618.993619
ATATACG307.744623E-418.993619
Produced by FastQC (version 0.11.5)