FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n02_S_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n02_S_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11072457
Sequences flagged as poor quality0
Sequence length25
%GC8

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT242076621.86295236910832No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA3667583.3123452184099698No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG3122172.819762587472681No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2656722.3993951839234957No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT2144821.9370768385011565No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT1277141.1534386631621147No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA1162601.0499927884118223No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1045250.9440090848851344No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT989320.8934963576738206No Hit
TTTTTTTTTTTTTTTTTTTTTTATT878740.7936269249002277No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC844140.7623782146997725No Hit
TTTTTTTTTTTTTTTTTTTTTTACA589200.5321312153210439No Hit
TTTTTTATTTTTTTTTTTTTTTTTT507650.4584799922907806No Hit
TTTTTTTTTTTTTTTTTTTTTTACC481200.4345918886837854No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG455180.4110921360995125No Hit
TTTTTATTTTTTTTTTTTTTTTTTT447900.4045172629706307No Hit
TTTTATTTTTTTTTTTTTTTTTTTT433520.39153008225726227No Hit
TTATTTTTTTTTTTTTTTTTTTTTT417810.3773417227991944No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC393970.3558108195859329No Hit
TTTTTTTTATTTTTTTTTTTTTTTT364080.3288159077971583No Hit
TTTATTTTTTTTTTTTTTTTTTTTT341850.3087390630643226No Hit
TTTTTTTATTTTTTTTTTTTTTTTT328270.2964743958816006No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC312000.2817802769520803No Hit
TTTTTTTTTTATTTTTTTTTTTTTT303570.2741667906228943No Hit
TTTTTTTTTTTTTTTTTTTTTAATT301140.27197215577355593No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA299260.270274248976537No Hit
TTTTTTTTTATTTTTTTTTTTTTTT296520.2677996401340732No Hit
TTTTTTTTTTTATTTTTTTTTTTTT256250.2314301152851621No Hit
TTTTTTTTTTTTATTTTTTTTTTTT253950.2293528888845538No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA248820.22471977086928405No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT245330.22156780559183928No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA239340.21615798553112467No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC225070.20327015042822025No Hit
TTTTTTTTTTTTTTATTTTTTTTTT223970.20227669432358147No Hit
TTTTTTTTTTTTTATTTTTTTTTTT215820.19491608773012167No Hit
TTTTTTTTTTTTTTTTTTTTTATTT212340.19177315387181004No Hit
TTTTTTTTTTTTTTTATTTTTTTTT208840.18861215717523222No Hit
TTTTTTTTTTTTTTTTTTTTTACCA205480.1855776003465175No Hit
TTTTTTTTTTTTTTTTATTTTTTTT196890.1778196113112022No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG190680.17221110002955983No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT180930.16340546637480732No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT176480.15938648486058696No Hit
TTTTTTTTTTTTTTTTTTTTTTAAG173450.15664996486326385No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT169250.15285676882737048No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT163870.1479978653337737No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT160590.14503555985812364No Hit
TTTTTTTTTTTTTTTTTTTTTTACT155960.14085401279950782No Hit
TTTTTTTTTTTTTTTTTATTTTTTT152530.13775623603686155No Hit
TTTTTTTTTTTTTTTTTTTTTTATA142290.12850806284458816No Hit
TTTTTTTTTTTTTTTTTTATTTTTT139980.1264218050248468No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT139070.12559994588373655No Hit
TTTTTTTTTTTTTTTTTTTTTACCC130190.11758004569356197No Hit
TATTTTTTTTTTTTTTTTTTTTTTT129440.11690268925858101No Hit
TTTTTTTTTTTTTTTTTTTTTACAA128900.1164149926253947No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT118350.10688684543999584No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT116040.10480058762025449No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA114220.103156869338034No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT113370.10238919871172225No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT112830.10190150207853596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA502.1431515E-719.0277041
GCCCCAA654.802132E-1019.0277021
CCCTCAC307.710331E-419.0060353
CCTCAAC552.8461727E-818.9987354
AAGAGGG250.006037626418.9982212
CTGAAAA502.1784035E-718.9982212
CAAAAAG451.6717859E-618.9982215
GTAAAGT250.006037941718.99804913
CTACACC307.733326E-418.997625
GTCTGAA250.0060399918.99693310
ATAGGTA250.006042038618.9958179
ACCCGTG359.9651785E-518.99538818
AACGTTT307.7443745E-418.99358719
TTGCAAT250.006046138218.99358719
AACGGTT250.006046138218.99358719
GTAAAAG1400.018.31954813
CCTAAAC2650.017.9233344
CCCCATC751.72804E-1017.72902918
TCCCCTA2750.017.6438711
GTAAAGG1250.017.47820513
Produced by FastQC (version 0.11.5)