FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n02_S_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n02_S_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10041854
Sequences flagged as poor quality0
Sequence length25
%GC8

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT214783621.388839152610664No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA3283883.270192934491977No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG2905982.8938680048524903No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2328322.318615665991559No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1945571.9374609509359528No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT1189391.1844326754800458No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA1012901.0086782779355286No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC993730.9895881776413No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT881980.8783039466616424No Hit
TTTTTTTTTTTTTTTTTTTTTTATT782610.7793481163936461No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC777630.7743888728117339No Hit
TTTTTTTTTTTTTTTTTTTTTTACA511700.5095672572017079No Hit
TTTTTTATTTTTTTTTTTTTTTTTT464500.4625639846984431No Hit
TTTTTTTTTTTTTTTTTTTTTTACC439560.4377279335070994No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG439550.4377179751866538No Hit
TTTTTATTTTTTTTTTTTTTTTTTT401570.3998962741342386No Hit
TTTTATTTTTTTTTTTTTTTTTTTT386850.3852376264383051No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC371980.3704296039356876No Hit
TTATTTTTTTTTTTTTTTTTTTTTT364240.3627218639107878No Hit
TTTTTTTTATTTTTTTTTTTTTTTT335130.3337331930936259No Hit
TTTATTTTTTTTTTTTTTTTTTTTT312180.31087884767095797No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC290490.2892792506244365No Hit
TTTTTTTATTTTTTTTTTTTTTTTT285880.2846884648990117No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA273470.27233018922601343No Hit
TTTTTTTTTTATTTTTTTTTTTTTT266820.2657079061296848No Hit
TTTTTTTTTTTTTTTTTTTTTAATT264490.26338761746585837No Hit
TTTTTTTTTATTTTTTTTTTTTTTT256370.25530146126402553No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT231840.2308737012109517No Hit
TTTTTTTTTTTATTTTTTTTTTTTT228280.2273285391323156No Hit
TTTTTTTTTTTTATTTTTTTTTTTT226640.22569537457923605No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA213200.21231139190034032No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC207730.2068641906165933No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA201870.20102861483546763No Hit
TTTTTTTTTTTTTTATTTTTTTTTT197260.19643782911004282No Hit
TTTTTTTTTTTTTATTTTTTTTTTT196360.1955415802699382No Hit
TTTTTTTTTTTTTTTATTTTTTTTT185230.18445796961397765No Hit
TTTTTTTTTTTTTTTTTTTTTATTT184610.18384055374635003No Hit
TTTTTTTTTTTTTTTTATTTTTTTT177350.17661081310283938No Hit
TTTTTTTTTTTTTTTTTTTTTACCA173250.17252790172014051No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG170810.17009807153141243No Hit
TTTTTTTTTTTTTTTTTTTTTTAAG165840.16514878626994578No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT152570.15193409503862532No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT150630.15000218087217757No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT147280.14666614352289925No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT147230.14661635192067124No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT142360.14176664986366064No Hit
TTTTTTTTTTTTTTTTTTTTTTACT140700.14011356866968988No Hit
TTTTTTTTTTTTTTTTTATTTTTTT136070.13550286630337388No Hit
TTTTTTTTTTTTTTTTTTTTTTATA128080.1275461682673339No Hit
TTTTTTTTTTTTTTTTTTATTTTTT121570.12106330165724377No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT117650.11715964004256584No Hit
TTTTTTTTTTTTTTTTTTTTTACCC113340.11286760393050925No Hit
TATTTTTTTTTTTTTTTTTTTTTTT113320.11284768728961803No Hit
TTTTTTTTTTTTTTTTTTTTTACAA108380.1079282769894882No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT101990.10156491022474536No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT100470.10005124551701311No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA359.844627E-519.0255091
CCCTCAC451.6656304E-619.0055073
CCTCACC502.1764208E-718.9998254
TCTGAAA250.006036726318.9986911
CTGAAAA250.006036918.99859412
GGCAAAA401.2872279E-518.998513
AGGGTGG250.006037073718.99849913
GCAAAAA401.2872832E-518.99840514
CTCAACC654.8930815E-1018.9981235
CCAGTGG250.00603846318.99774610
GAGTTTG401.2876702E-518.99774410
TCAACCC654.9112714E-1018.9970826
AAAACGG552.8519935E-818.99528317
TGGGAGT250.0060438518.9948118
AAACGGT552.852721E-818.9948118
TTAACAG401.2904935E-518.9929219
GGGTATG250.006047327518.99291819
CCCATAC250.006047327518.99291819
GGTTAAG307.746138E-418.99291819
CCCCTTA307.746138E-418.99291819
Produced by FastQC (version 0.11.5)