FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n02_S_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n02_S_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6271747
Sequences flagged as poor quality0
Sequence length25
%GC7

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT138856122.139939637233454No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA2165573.4528975738338934No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1660732.6479543897418054No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1586572.5297098240729414No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1220141.945454751283813No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA700401.1167542313170475No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT677711.0805761138005088No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT587170.9362144231902212No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC585180.9330414635666905No Hit
TTTTTTTTTTTTTTTTTTTTTTATT484710.7728468638801916No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC483940.7716191357846546No Hit
TTTTTTTTTTTTTTTTTTTTTTACA347000.5532748690277206No Hit
TTTTTTATTTTTTTTTTTTTTTTTT314020.5006898396890053No Hit
TTTTTATTTTTTTTTTTTTTTTTTT285850.45577412481721596No Hit
TTTTTTTTTTTTTTTTTTTTTTACC280500.44724380623134197No Hit
TTTTATTTTTTTTTTTTTTTTTTTT271100.4322559567533576No Hit
TTATTTTTTTTTTTTTTTTTTTTTT259810.4142545928590551No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG242690.386957573384258No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC226040.36040994638336016No Hit
TTTTTTTTATTTTTTTTTTTTTTTT221220.3527246873957129No Hit
TTTATTTTTTTTTTTTTTTTTTTTT219960.35071567778483415No Hit
TTTTTTTATTTTTTTTTTTTTTTTT201900.32191987336223865No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC184940.2948779662189817No Hit
TTTTTTTTTTATTTTTTTTTTTTTT179870.28679409421330293No Hit
TTTTTTTTTTTTTTTTTTTTTAATT177890.28363707911049346No Hit
TTTTTTTTTATTTTTTTTTTTTTTT173830.2771636036976619No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA169110.2696377899172272No Hit
TTTTTTTTTTTATTTTTTTTTTTTT151550.2416392115306947No Hit
TTTTTTTTTTTTATTTTTTTTTTTT149710.2387054197179829No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA141140.2250409654598631No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA140860.2245945188796678No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT133670.21313040848108192No Hit
TTTTTTTTTTTTTTATTTTTTTTTT131600.2098298926917811No Hit
TTTTTTTTTTTTTATTTTTTTTTTT126240.20128362958518575No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC125480.20007184601036998No Hit
TTTTTTTTTTTTTTTTTTTTTACCA124200.19803094735804874No Hit
TTTTTTTTTTTTTTTATTTTTTTTT121970.19447531923720776No Hit
TTTTTTTTTTTTTTTTTTTTTATTT115780.18460566091074784No Hit
TTTTTTTTTTTTTTTTATTTTTTTT114520.1825966512998691No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG105540.16827847169217763No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT104000.1658230155011036No Hit
TATTTTTTTTTTTTTTTTTTTTTTT92600.14764626187886723No Hit
TTTTTTTTTTTTTTTTTTTTTTAAG92560.1475824837959822No Hit
TTTTTTTTTTTTTTTTTATTTTTTT90210.14383552142648612No Hit
TTTTTTTTTTTTTTTTTTTTTTACT88730.14147573235973965No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT86110.13729826793076952No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT84600.13489064530185926No Hit
TTTTTTTTTTTTTTTTTTTTTTATA83490.13312080350179942No Hit
TTTTTTTTTTTTTTTTTTTTTACAA79570.12687055137906553No Hit
TTTTTTTTTTTTTTTTTTATTTTTT78800.1256428232835285No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT78410.12502098697539937No Hit
TTTTTTTTTTTTTTTTTTTTTACCC77830.12409620477356628No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT71620.11419465740566384No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA67710.1079603498036512No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT64120.10223626686471889No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAAC250.006020962319.0071583
CCTAACC250.00603596218.998974
CCTAAAC1400.018.998974
CTAACCC250.006037630618.9980625
GGGGCAA307.732017E-418.9979111
GTATGAA250.00603818718.99775910
GGCAAAA401.2878896E-518.99715213
TGTATGA250.006041525418.9959419
TTACCAG706.548362E-1118.9927619
TCCCCTA1550.018.415921
CTAAACC1400.018.319565
CCCCTAA1650.017.8637622
CCCTAAA1500.017.7400133
GGTAAAG555.9255035E-717.27027712
AAGGGTT453.5462064E-516.88635816
TAAAGGG453.5462064E-516.88635814
GTTTAAG453.5532463E-516.88245419
GTGAAAA402.765786E-416.6226412
TTAACAG402.7708375E-416.61866419
GTAAAAG1150.016.51926213
Produced by FastQC (version 0.11.5)