FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n02_S_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n02_S_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10278302
Sequences flagged as poor quality0
Sequence length25
%GC8

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT223660521.76045226147276No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA3435443.342419788793908No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG3137983.053014009512466No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2447932.381648252795063No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1939781.88725725319221No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT1245611.2118830522784796No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA1072931.043878648438234No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1018000.9904359688983647No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT880030.856201734488829No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC824300.8019807162700611No Hit
TTTTTTTTTTTTTTTTTTTTTTATT779280.7581797071150468No Hit
TTTTTTTTTTTTTTTTTTTTTTACA536570.5220414811707226No Hit
TTTTTTATTTTTTTTTTTTTTTTTT471840.45906415281434615No Hit
TTTTTTTTTTTTTTTTTTTTTTACC463820.4512613075583885No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG447750.43562642934601453No Hit
TTTTTATTTTTTTTTTTTTTTTTTT427810.416226337774469No Hit
TTTTATTTTTTTTTTTTTTTTTTTT402330.3914362508515512No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC386160.37570408030431485No Hit
TTATTTTTTTTTTTTTTTTTTTTTT385020.37459494768688445No Hit
TTTTTTTTATTTTTTTTTTTTTTTT332190.3231954071791236No Hit
TTTATTTTTTTTTTTTTTTTTTTTT313100.30462230045390765No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC299500.29139054291263283No Hit
TTTTTTTATTTTTTTTTTTTTTTTT294120.28615621529704033No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA281830.2741989873424618No Hit
TTTTTTTTTTATTTTTTTTTTTTTT274870.2674274408360447No Hit
TTTTTTTTTATTTTTTTTTTTTTTT270750.26341899663971735No Hit
TTTTTTTTTTTTTTTTTTTTTAATT264970.2577954996846755No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA250470.2436881111296399No Hit
TTTTTTTTTTTTATTTTTTTTTTTT231420.2251539213383689No Hit
TTTTTTTTTTTATTTTTTTTTTTTT230530.22428801955809435No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC228680.22248811136314153No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT225930.21981257215442784No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA217780.2118832468631492No Hit
TTTTTTTTTTTTTTATTTTTTTTTT200240.19481817132829918No Hit
TTTTTTTTTTTTTATTTTTTTTTTT195440.19014813925490806No Hit
TTTTTTTTTTTTTTTATTTTTTTTT188160.18306525727693154No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG186920.18185883232463884No Hit
TTTTTTTTTTTTTTTTTTTTTATTT185630.18060376120491498No Hit
TTTTTTTTTTTTTTTTTTTTTACCA185580.1805551150374838No Hit
TTTTTTTTTTTTTTTTATTTTTTTT179720.17485378421455217No Hit
TTTTTTTTTTTTTTTTTTTTTTAAG167410.16287709779300122No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT155690.15147443614713793No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT154150.14997613419025826No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT153900.1497329033531025No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT145190.14125874098659488No Hit
TTTTTTTTTTTTTTTTTTTTTTACT139640.1358590164017364No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT138940.13517797005770021No Hit
TTTTTTTTTTTTTTTTTATTTTTTT137300.13358237576595822No Hit
TTTTTTTTTTTTTTTTTTTTTTATA135790.13211326150953726No Hit
TTTTTTTTTTTTTTTTTTTTTACCC124820.12144029237514133No Hit
TTTTTTTTTTTTTTTTTTATTTTTT123090.1197571349820233No Hit
TATTTTTTTTTTTTTTTTTTTTTTT122480.11916365173936316No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT116880.11371528098707354No Hit
TTTTTTTTTTTTTTTTTTTTTACAA116340.11318990237881704No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA107370.10446277994166742No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT106390.10350931506001673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA451.6480608E-619.0265851
CCTCAAC502.1760934E-719.0001034
CTGAAAA250.00603923418.9973312
AAGGGTT900.018.99732816
TCTGAAA250.006039403418.99723611
GGGGCAA451.6725917E-618.99723611
TCTGCCG307.735338E-418.9968649
CCCCATC250.006041949618.9958518
AAACGGT307.7381224E-418.9958518
CTAAACC1300.018.2680855
CCTAAAC1250.018.2400974
GGTAAAG1050.018.09269312
GTAAAGG1050.018.09260613
GTAAAAG1550.017.15879413
GGGCAAA504.573454E-617.09759712
GGCAAAA504.573665E-617.09751313
TAAAAGG1400.016.961914
AAGGTTG855.638867E-1116.76120817
TTTAACG1250.016.7152119
CCCTAAA1600.016.6300263
Produced by FastQC (version 0.11.5)