FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n02_S_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n02_S_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11143121
Sequences flagged as poor quality0
Sequence length25
%GC7

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT251418422.56265547147877No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4272603.8342938212732323No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG3341762.998944371150596No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA3142722.8203229597883754No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT2064221.8524612628724033No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA1330321.1938486533530417No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT1196211.0734963750281452No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1180171.0591018440883841No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC986580.8853713425529526No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT940750.8442428292755683No Hit
TTTTTTTTTTTTTTTTTTTTTTATT754250.6768749975881981No Hit
TTTTTTTTTTTTTTTTTTTTTTACA669660.6009626925885486No Hit
TTTTTTTTTTTTTTTTTTTTTTACC572430.5137070664493368No Hit
TTTTTTATTTTTTTTTTTTTTTTTT556700.4995907340501822No Hit
TTTTTATTTTTTTTTTTTTTTTTTT498060.4469663391432257No Hit
TTTTATTTTTTTTTTTTTTTTTTTT475490.426711690557789No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC451950.40558654976464853No Hit
TTATTTTTTTTTTTTTTTTTTTTTT447360.4014674165343803No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG425960.3822627430860708No Hit
TTTTTTTTATTTTTTTTTTTTTTTT393160.35282754266062444No Hit
TTTATTTTTTTTTTTTTTTTTTTTT373900.33554333655714585No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC358280.3215257197691742No Hit
TTTTTTTATTTTTTTTTTTTTTTTT343490.3082529571383098No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA332390.29829165455530815No Hit
TTTTTTTTTTATTTTTTTTTTTTTT318600.28591630657156103No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA316780.2842830119138076No Hit
TTTTTTTTTATTTTTTTTTTTTTTT299030.26835390192747616No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC276150.24782105480143313No Hit
TTTTTTTTTTTATTTTTTTTTTTTT272110.24419549962707934No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA269670.2420058078881132No Hit
TTTTTTTTTTTTATTTTTTTTTTTT266810.23943920199735783No Hit
TTTTTTTTTTTTTTTTTTTTTAATT265530.23829051124904774No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT239330.21477824749457536No Hit
TTTTTTTTTTTTTTTTTTTTTACCA232390.20855018984358153No Hit
TTTTTTTTTTTTTATTTTTTTTTTT230370.20673741225640466No Hit
TTTTTTTTTTTTTTATTTTTTTTTT225760.20260033073319406No Hit
TTTTTTTTTTTTTTTATTTTTTTTT217930.19557357404626588No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG214770.19273774376137529No Hit
TTTTTTTTTTTTTTTTATTTTTTTT202940.182121328485978No Hit
TTTTTTTTTTTTTTTTTTTTTATTT175290.15730781349318562No Hit
TTTTTTTTTTTTTTTTTATTTTTTT167700.15049643632156556No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT166720.14961696996739063No Hit
TTTTTTTTTTTTTTTTTTTTTTAAG162930.1462157684548162No Hit
TTTTTTTTTTTTTTTTTTTTTACCC160460.14399915427643656No Hit
TTTTTTTTTTTTTTTTTTTTTTATA158960.14265303230576065No Hit
TTTTTTTTTTTTTTTTTTTTTTACT151110.13560832732589012No Hit
TTTTTTTTTTTTTTTTTTTTTACAA149850.13447758487052236No Hit
TATTTTTTTTTTTTTTTTTTTTTTT148080.1328891609451248No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT147370.13225199654567152No Hit
TTTTTTTTTTTTTTTTTTATTTTTT138780.12454320472693421No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT137740.12360989349393227No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA133710.11999331246604968No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT131630.11812669000004576No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC126310.1133524440773819No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT121880.10937689719065241No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA120860.10846153425059281No Hit
TTTTTTTTTTTTTTTTTTTTTAACA118520.1063615839763384No Hit
TTTTTTATTTTTTTTTTTTTTTTTA113340.10171297610427096No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA307.645423E-419.0299431
TCACCTA401.2690249E-519.0299421
CGCCTAA250.006005087419.0160052
CGCTAAA502.1676897E-719.0071243
GCCTAAA401.28222255E-519.0071223
GCTAAAA307.7260064E-419.0002984
GCTAAAC451.6700415E-619.0002964
CTAAACC6050.018.998255
CTCAACC900.018.998255
AAGTCGG250.006038826418.99756811
GGCAAAA502.179404E-718.99739813
CAAAAAG502.1794949E-718.99731315
GGATTAA307.735108E-418.9969716
AAACGCA250.00604195818.9958638
AAACGGT401.2898394E-518.99407218
TTACAAG250.00604634518.99347519
AACGGTT359.972896E-518.99347519
CCCTAAA6550.018.5718443
CCTAAAC5850.018.513114
GTAAAAG1100.018.13387913
Produced by FastQC (version 0.11.5)