FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n02_S_21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n02_S_21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8477857
Sequences flagged as poor quality0
Sequence length25
%GC7

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT187095122.06867844078993No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA3100923.6576696209903043No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG2536252.9916168673286188No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2259592.6652843991117092No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1496281.764927150811815No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA957161.1290117302049327No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT891661.0517516395947704No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC882881.0413952488229041No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC739430.872189752669808No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT674860.7960266373919731No Hit
TTTTTTTTTTTTTTTTTTTTTTATT539660.6365523740256529No Hit
TTTTTTTTTTTTTTTTTTTTTTACA480540.5668177701039308No Hit
TTTTTTATTTTTTTTTTTTTTTTTT436080.5143752719584678No Hit
TTTTTTTTTTTTTTTTTTTTTTACC422700.498592981693369No Hit
TTTTTATTTTTTTTTTTTTTTTTTT394980.46589603952980096No Hit
TTTTATTTTTTTTTTTTTTTTTTTT370550.4370797950472625No Hit
TTATTTTTTTTTTTTTTTTTTTTTT347920.4103867286272934No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC334130.39412082558127604No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG333320.39316539545311985No Hit
TTTTTTTTATTTTTTTTTTTTTTTT320320.37783133166789673No Hit
TTTATTTTTTTTTTTTTTTTTTTTT301240.3553256442046616No Hit
TTTTTTTATTTTTTTTTTTTTTTTT273900.3230769285209694No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC267690.31575196420510515No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA248380.2929749817672084No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA247450.2918780064348809No Hit
TTTTTTTTTTATTTTTTTTTTTTTT246940.2912764393171529No Hit
TTTTTTTTTATTTTTTTTTTTTTTT234190.2762372613739533No Hit
TTTTTTTTTTTATTTTTTTTTTTTT213540.25187969082281053No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC209730.24738563059037208No Hit
TTTTTTTTTTTTATTTTTTTTTTTT208820.24631224612540648No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA196140.2313556362179735No Hit
TTTTTTTTTTTTTTTTTTTTTAATT191970.22643694037302117No Hit
TTTTTTTTTTTTTATTTTTTTTTTT183720.21670570758624497No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT177310.2091448345967619No Hit
TTTTTTTTTTTTTTATTTTTTTTTT175950.20754065561615392No Hit
TTTTTTTTTTTTTTTTTTTTTACCA169880.20038082737182283No Hit
TTTTTTTTTTTTTTTATTTTTTTTT167060.19705451507379754No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG163460.19280815894865883No Hit
TTTTTTTTTTTTTTTTATTTTTTTT156400.1844805827699146No Hit
TTTTTTTTTTTTTTTTTATTTTTTT129830.1531401154796548No Hit
TTTTTTTTTTTTTTTTTTTTTATTT125360.14786755662427425No Hit
TTTTTTTTTTTTTTTTTTTTTTAAG122670.1446945849641012No Hit
TTTTTTTTTTTTTTTTTTTTTACCC118390.13964613934865852No Hit
TTTTTTTTTTTTTTTTTTTTTTATA118070.1392686854708684No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT116960.13795939233228396No Hit
TATTTTTTTTTTTTTTTTTTTTTTT116240.13711012110725623No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT107730.1270722070447756No Hit
TTTTTTTTTTTTTTTTTTATTTTTT107680.1270132298763709No Hit
TTTTTTTTTTTTTTTTTTTTTTACT107130.12636448102391912No Hit
TTTTTTTTTTTTTTTTTTTTTACAA106540.12566855043674363No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT99950.11789535964100362No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA99620.11750611032953256No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT96520.11384952588844091No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC95420.1125520281835374No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA89950.10609992596006279No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT89550.10562810861282515No Hit
TTTTTTTTTTTTTTTTTTTTTAACA85860.10127559358455798No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA250.00598466219.0271631
GAAGGTG307.729693E-418.99887311
GGTAAAG1000.018.99831212
GTAAAGT307.7315327E-418.99820113
GTAAAAG950.018.998213
CTCAACC401.28788815E-518.9973055
CTGTAAA250.006039811818.9969676
AGGAAGG250.006040223418.9967449
CTAAACC1550.018.3844875
CCTAAAC1700.017.3218884
CCCTAAA1550.017.1652363
GTAAGTG453.5471E-516.8859949
GTAAAGG855.638867E-1116.76311713
CCTCAAC402.764934E-416.6234264
TGTGAAA1050.016.28474811
GTGAAAA1050.016.28426712
TTCAAAG350.002175644716.2808119
TGTTAAG350.002175644716.2808119
CCAAAAC3100.016.2403964
GTGTGAA851.024091E-915.64511510
Produced by FastQC (version 0.11.5)