FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n02_S_20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n02_S_20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11276232
Sequences flagged as poor quality0
Sequence length25
%GC8

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT259399323.00407618431405No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4004193.5510000148985936No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG3293312.9205766607143238No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2911942.5823697135709875No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT2169151.9236478994046946No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT1275801.1314063066456952No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA1229081.089974026784834No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1141891.0126520986797718No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT972240.8622029060771363No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC931180.8257900334083228No Hit
TTTTTTTTTTTTTTTTTTTTTTATT823000.7298537312818679No Hit
TTTTTTTTTTTTTTTTTTTTTTACA631230.5597880568615474No Hit
TTTTTTATTTTTTTTTTTTTTTTTT535310.47472418091433377No Hit
TTTTTTTTTTTTTTTTTTTTTTACC521820.4627609648329336No Hit
TTTTTATTTTTTTTTTTTTTTTTTT468550.41552000703781194No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG463550.41108590174448345No Hit
TTTTATTTTTTTTTTTTTTTTTTTT461860.4095871741553384No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC424310.3762870434024415No Hit
TTATTTTTTTTTTTTTTTTTTTTTT420720.3731033558018317No Hit
TTTTTTTTATTTTTTTTTTTTTTTT390490.34629475519836767No Hit
TTTATTTTTTTTTTTTTTTTTTTTT358740.31813818658573184No Hit
TTTTTTTATTTTTTTTTTTTTTTTT340830.3022552214250292No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC333980.2961804971731692No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA323030.2864698065807798No Hit
TTTTTTTTTTATTTTTTTTTTTTTT301430.2673144717136008No Hit
TTTTTTTTTATTTTTTTTTTTTTTT294190.26089388724886114No Hit
TTTTTTTTTTTTTTTTTTTTTAATT288880.2561848674273463No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA271360.2406477624795233No Hit
TTTTTTTTTTTATTTTTTTTTTTTT262080.23241806305510565No Hit
TTTTTTTTTTTTATTTTTTTTTTTT258620.22934966219212233No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT255120.2262457884867924No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC252800.22418836363068798No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA247150.2191778246492268No Hit
TTTTTTTTTTTTTATTTTTTTTTTT224230.19885188598460904No Hit
TTTTTTTTTTTTTTTTTTTTTACCA219310.1944887263759738No Hit
TTTTTTTTTTTTTTATTTTTTTTTT218340.1936285099490681No Hit
TTTTTTTTTTTTTTTATTTTTTTTT211200.18729660759019504No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG202800.17984731069740317No Hit
TTTTTTTTTTTTTTTTTTTTTATTT200130.17747949847076577No Hit
TTTTTTTTTTTTTTTTATTTTTTTT193700.17177723906354533No Hit
TTTTTTTTTTTTTTTTTTTTTTAAG174760.15498084821241706No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT171000.15164640103183402No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT167610.14864007764295734No Hit
TTTTTTTTTTTTTTTTTATTTTTTT159490.14143909064659188No Hit
TTTTTTTTTTTTTTTTTTTTTTACT158150.14025075042797983No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT153520.13614476892635768No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT152530.13526681607827865No Hit
TTTTTTTTTTTTTTTTTTTTTTATA145950.12943153351225833No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT145100.1286777356123925No Hit
TTTTTTTTTTTTTTTTTTTTTACCC139600.12380021978973119No Hit
TATTTTTTTTTTTTTTTTTTTTTTT138260.12261187957111913No Hit
TTTTTTTTTTTTTTTTTTTTTACAA135860.12048350903032148No Hit
TTTTTTTTTTTTTTTTTTATTTTTT133670.11854137091184361No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT127450.11302534392694298No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA120980.10728761167737592No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT114550.1015853522701555No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA307.6517346E-419.027611
TCCGCTA401.2703684E-519.027611
TCACCTA706.366463E-1119.027611
CCGCTAA250.006006265519.0153622
CCACTAA307.6849444E-419.015362
GCCTAAA401.2829789E-519.0058273
CCTACAC950.018.9995044
CCGAAAC502.1768574E-718.9995044
GCTAAAC307.7281846E-418.9995024
CGCAACC250.00603775218.9981565
CGAAACC451.6718477E-618.9981545
CTCAACC1300.018.9981545
GGGTAGA250.00603837118.99781811
GGGGCAA451.6721315E-618.99781611
GTAAAGT307.7330275E-418.99773213
GGGGGCA451.6722734E-618.99764810
GCAACCC250.006039453718.9972296
GTCTAAA307.7344116E-418.9972276
GTAAGTG552.8507202E-818.9960489
AACGTTT250.006045180418.99411219
Produced by FastQC (version 0.11.5)