FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n02_S_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n02_S_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7861728
Sequences flagged as poor quality0
Sequence length25
%GC9

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT161516520.544656340183735No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA2443413.1079808408533087No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG2257382.871353473434848No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1733342.2047824600393198No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1490121.8954102711261442No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT930451.183518432588866No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA764100.971923729744911No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC738630.9395262720867473No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT677330.8615535922891252No Hit
TTTTTTTTTTTTTTTTTTTTTTATT612390.7789508871332105No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC587850.7477363755143908No Hit
TTTTTTTTTTTTTTTTTTTTTTACA381810.4856565884752055No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG347810.4424090988647788No Hit
TTTTTTATTTTTTTTTTTTTTTTTT345220.4391146577444552No Hit
TTTTTTTTTTTTTTTTTTTTTTACC331920.4221972573968471No Hit
TTTTTATTTTTTTTTTTTTTTTTTT296880.3776269033983369No Hit
TTTTATTTTTTTTTTTTTTTTTTTT284910.3624012430854896No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC283310.36036606710382246No Hit
TTATTTTTTTTTTTTTTTTTTTTTT263910.3356895583261085No Hit
TTTTTTTTATTTTTTTTTTTTTTTT236970.30142228273478805No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC219800.27958230048152266No Hit
TTTATTTTTTTTTTTTTTTTTTTTT217920.27719096870306376No Hit
TTTTTTTATTTTTTTTTTTTTTTTT212150.2698516153191766No Hit
TTTTTTTTTTTTTTTTTTTTTAATT207720.2642167218199358No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA202520.25760239987951755No Hit
TTTTTTTTTATTTTTTTTTTTTTTT193800.24651069077943172No Hit
TTTTTTTTTTATTTTTTTTTTTTTT189070.2404942017836282No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT173600.22081659401088413No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA168860.21478738516519524No Hit
TTTTTTTTTTTATTTTTTTTTTTTT164950.20981392385999614No Hit
TTTTTTTTTTTTATTTTTTTTTTTT164360.20906345271675641No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA157460.2002867562958169No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC155520.1978191054180455No Hit
TTTTTTTTTTTTTTTTTTTTTATTT152110.19348163660711742No Hit
TTTTTTTTTTTTTTATTTTTTTTTT140630.17887924893865573No Hit
TTTTTTTTTTTTTATTTTTTTTTTT136560.17370227003528996No Hit
TTTTTTTTTTTTTTTTTTTTTACCA135430.17226492699823753No Hit
TTTTTTTTTTTTTTTATTTTTTTTT133620.1699626341689766No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG128480.1634246313278709No Hit
TTTTTTTTTTTTTTTTTTTTTTAAG128440.16337375192832926No Hit
TTTTTTTTTTTTTTTTATTTTTTTT125150.1591889213160262No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT123520.1571155857847028No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT122050.15524576785154612No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT118240.15039950504520125No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT117480.14943279645390936No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT115520.14693970587636712No Hit
TTTTTTTTTTTTTTTTTTTTTTACT107260.13643310987101054No Hit
TTTTTTTTTTTTTTTTTATTTTTTT98590.12540500002035176No Hit
TTTTTTTTTTTTTTTTTTTTTTATA95470.12143640685610085No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT92130.11718797699437071No Hit
TTTTTTTTTTTTTTTTTTTTTACCC89630.11400801452301582No Hit
TTTTTTTTTTTTTTTTTTATTTTTT87730.11159124304478608No Hit
TATTTTTTTTTTTTTTTTTTTTTTT85700.10900911351804592No Hit
TTTTTTTTTTTTTTTTTTTTTACAA84570.10757177048099349No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT82500.10493876155471164No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT79940.10168247998404423No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG78700.10010521859825218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCCTA950.019.0259761
TCTGAAA307.732634E-418.99777211
AAGTCGG250.00603829518.99777211
CTGAAAA307.7329646E-418.99765212
AGTCGGT250.00603851718.99765212
GGTAAAG850.018.99765212
GTCTAAA359.956576E-518.9974126
GTCTGAA250.006039626418.99704710
GTGACTT250.006040292318.9966878
ACCCGTG250.00604295618.99523418
GGTTCAA250.00604295618.99523418
CCCCAGC250.00604517618.99402819
GGGTAAT359.970195E-518.99402619
CCCATCA359.970195E-518.99402619
TACCCAG307.7428954E-418.99402619
GTAAAAG1300.018.26697313
GTGAAAA1050.018.09300212
AAGGTTG950.017.99628817
AAGTGGG900.017.9423411
TAAGTGG853.6379788E-1217.87957610
Produced by FastQC (version 0.11.5)