FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n02_S_19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n02_S_19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8524946
Sequences flagged as poor quality0
Sequence length25
%GC8

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT187793222.028667395664442No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA2985403.501957666359411No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG2586093.0335558723773732No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2139692.5099161918444994No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1620601.9010091090313066No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT971721.1398547275255468No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA917051.0757252890516842No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC901381.0573439409469574No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT728910.855031808999142No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC721980.8469027252489341No Hit
TTTTTTTTTTTTTTTTTTTTTTATT608370.713635018919768No Hit
TTTTTTTTTTTTTTTTTTTTTTACA464150.5444609267906213No Hit
TTTTTTATTTTTTTTTTTTTTTTTT413520.485070521267818No Hit
TTTTTTTTTTTTTTTTTTTTTTACC399080.4681319975516561No Hit
TTTTTATTTTTTTTTTTTTTTTTTT380000.44575062410952515No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG370900.4350760696900602No Hit
TTTTATTTTTTTTTTTTTTTTTTTT362860.4256449249062692No Hit
TTATTTTTTTTTTTTTTTTTTTTTT330740.3879672668894325No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC327250.38387339931537395No Hit
TTTTTTTTATTTTTTTTTTTTTTTT302120.35439520672623614No Hit
TTTATTTTTTTTTTTTTTTTTTTTT289170.3392044946677668No Hit
TTTTTTTATTTTTTTTTTTTTTTTT262150.3075092792376632No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC257080.3015620274896756No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA245510.28799009401349873No Hit
TTTTTTTTTTATTTTTTTTTTTTTT234280.2748169900431041No Hit
TTTTTTTTTATTTTTTTTTTTTTTT228470.2680016976060611No Hit
TTTTTTTTTTTTTTTTTTTTTAATT209690.2459722325513851No Hit
TTTTTTTTTTTATTTTTTTTTTTTT205700.24129185099823505No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA203010.2381364057907229No Hit
TTTTTTTTTTTTATTTTTTTTTTTT201680.2365762786063396No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT199300.2337844720658641No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC194540.2282008589849132No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA182500.21407760236839038No Hit
TTTTTTTTTTTTTATTTTTTTTTTT174280.20443531255212644No Hit
TTTTTTTTTTTTTTATTTTTTTTTT169970.19937956205235788No Hit
TTTTTTTTTTTTTTTATTTTTTTTT161230.1891272976978388No Hit
TTTTTTTTTTTTTTTTTTTTTACCA156920.18407154719807023No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG150480.17651724714737196No Hit
TTTTTTTTTTTTTTTTATTTTTTTT149030.17481635660800665No Hit
TTTTTTTTTTTTTTTTTTTTTATTT140020.16424737470477818No Hit
TTTTTTTTTTTTTTTTTTTTTTAAG138560.16253475388583108No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT124950.14656984337496096No Hit
TTTTTTTTTTTTTTTTTATTTTTTT123400.14475165003977739No Hit
TTTTTTTTTTTTTTTTTTTTTTACT117770.1381475026352073No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT114860.1347339912768949No Hit
TTTTTTTTTTTTTTTTTTTTTTATA112730.13223544172596519No Hit
TATTTTTTTTTTTTTTTTTTTTTTT110950.1301474519603995No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT107820.12647587445128683No Hit
TTTTTTTTTTTTTTTTTTATTTTTT103990.1219831773714461No Hit
TTTTTTTTTTTTTTTTTTTTTACCC103080.12091572192949962No Hit
TTTTTTTTTTTTTTTTTTTTTACAA97980.11493327934276651No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT94950.11137900462947214No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT94500.1108511420482898No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA91870.10776607851826862No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAA250.005988146619.0252481
CCCGAAA250.006026655419.0041433
CCTCAAC1550.018.9989014
CCTTAAC307.729616E-418.9989014
GTAAAAG900.018.9986813
GGTAAAG654.8930815E-1018.99867812
CTAAACC5200.018.9976775
CTTAACC307.7329716E-418.9976775
CTCAACC1500.018.9976775
CTGTAAA359.958318E-518.9970076
AAACGGT401.2889757E-518.99544718
AAATTGC307.7430456E-418.99419
CCCATGC250.00604526918.99399819
CCCATCA401.2898236E-518.99399819
GTTTAAC250.00604526918.99399819
TTCCATG250.00604526918.99399819
CCCTCAA1550.018.3911083
TCAACCC1550.018.3842016
TCCCCTA5350.018.3140241
CCCTAAA6100.018.2252853
Produced by FastQC (version 0.11.5)