FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n02_S_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n02_S_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11834691
Sequences flagged as poor quality0
Sequence length25
%GC8

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT274453723.19060970835656No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4231813.57576720845521No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG3678313.1080743890989635No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA3102442.621479513068824No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT2296481.94046468978362No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT1418721.1987807708709928No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA1332961.1263158455087674No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1204711.0179479971213443No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT1017340.8596253167911186No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC980270.8283021500096622No Hit
TTTTTTTTTTTTTTTTTTTTTTATT872740.737442152059568No Hit
TTTTTTTTTTTTTTTTTTTTTTACA669900.5660477320447149No Hit
TTTTTTTTTTTTTTTTTTTTTTACC547190.4623610367182379No Hit
TTTTTTATTTTTTTTTTTTTTTTTT541090.4572066985103371No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG512550.43309115548517496No Hit
TTTTTATTTTTTTTTTTTTTTTTTT495580.41875195558549017No Hit
TTTTATTTTTTTTTTTTTTTTTTTT468900.3962080632269993No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC443340.37461054116241815No Hit
TTATTTTTTTTTTTTTTTTTTTTTT433710.3664734465817485No Hit
TTTTTTTTATTTTTTTTTTTTTTTT382490.3231939051049157No Hit
TTTATTTTTTTTTTTTTTTTTTTTT372220.31451602749915486No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC354380.2994417006747367No Hit
TTTTTTTATTTTTTTTTTTTTTTTT335830.28376744268185794No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA335270.2832942575349031No Hit
TTTTTTTTTTATTTTTTTTTTTTTT303730.25664379407962573No Hit
TTTTTTTTTATTTTTTTTTTTTTTT296730.25072897974269037No Hit
TTTTTTTTTTTTTTTTTTTTTAATT295400.24960516501867264No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA293110.24767017575701805No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA271190.2291483571476433No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC266570.22524457968526598No Hit
TTTTTTTTTTTATTTTTTTTTTTTT259710.2194480616350693No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT258560.21847634213685851No Hit
TTTTTTTTTTTTATTTTTTTTTTTT256080.21638080791462994No Hit
TTTTTTTTTTTTTTTTTTTTTACCA230970.1951635239145661No Hit
TTTTTTTTTTTTTATTTTTTTTTTT218100.18428871526937207No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG217580.18384932906148543No Hit
TTTTTTTTTTTTTTATTTTTTTTTT211720.17889778448799382No Hit
TTTTTTTTTTTTTTTTTTTTTATTT206700.17465601763493446No Hit
TTTTTTTTTTTTTTTATTTTTTTTT205080.17328716060267227No Hit
TTTTTTTTTTTTTTTTATTTTTTTT191480.16179552131948355No Hit
TTTTTTTTTTTTTTTTTTTTTTAAG191300.16164342609367663No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT182880.1545287494198201No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT181000.15294019928361458No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT171780.145149543828394No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT163090.13780672431582708No Hit
TTTTTTTTTTTTTTTTTTTTTTACT160280.13543234884628588No Hit
TTTTTTTTTTTTTTTTTATTTTTTT158350.13380155003624514No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT156540.13227214804340898No Hit
TTTTTTTTTTTTTTTTTTTTTTATA154820.1308187936634763No Hit
TTTTTTTTTTTTTTTTTTTTTACCC146360.12367031805055155No Hit
TTTTTTTTTTTTTTTTTTTTTACAA146000.12336612759893772No Hit
TATTTTTTTTTTTTTTTTTTTTTTT145020.12253805359176678No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT138260.11682603288924064No Hit
TTTTTTTTTTTTTTTTTTATTTTTT132350.11183223964191377No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA126350.10676239878168343No Hit
TTTTTTTTTTTTTTTTTTTTTAACA118640.10024765327628749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCCGA451.6478843E-619.026851
TCCACTA359.8395E-519.0268481
CCACTAA250.00600867219.0140512
CACTAAA307.7115267E-419.0056113
CGCAAAA250.006024102719.0056113
GCTAAAA359.949988E-518.9991844
CTACACC552.8461727E-818.9987035
GCAACCC250.006038238818.99796
ACCCGTG250.00604443218.9945318
GGGTGAT250.00604443218.9945318
AAATTGC250.0060469418.99316419
TTACACG250.0060469418.99316419
CCCATGC307.7455776E-418.99316419
TAACCAG451.6760714E-618.99316419
AACGGTT250.0060469418.99316419
TTTACGC552.8558134E-818.99316419
TACCAGT250.0060469418.99316419
CTAAACC5100.018.6261795
TCCCCTA4850.018.4383891
GTAAAAG1650.018.42189613
Produced by FastQC (version 0.11.5)