FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n02_S_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n02_S_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12884149
Sequences flagged as poor quality0
Sequence length25
%GC10

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT275871421.411689666116093No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG3848162.986739752854457No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA3518012.730494656651363No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT2695132.0918184041491603No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2512931.950404330157933No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT1683591.3067141648237692No Hit
TTTTTTTTTTTTTTTTTTTTTTATT1122330.8710936205410229No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT1086960.8436412835647895No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA1068340.8291894171667837No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC978650.7595767481422327No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC754850.5858749382671684No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG523560.4063597836380191No Hit
TTTTTTATTTTTTTTTTTTTTTTTT515750.40029807168482756No Hit
TTTTTTTTTTTTTTTTTTTTTTACA509860.3957265629262747No Hit
TTTTTATTTTTTTTTTTTTTTTTTT466210.36184772467316234No Hit
TTTTATTTTTTTTTTTTTTTTTTTT432660.3358079761418469No Hit
TTATTTTTTTTTTTTTTTTTTTTTT423220.328481143768207No Hit
TTTTTTTTTTTTTTTTTTTTTTACC413210.32071190732115873No Hit
TTTTTTTTATTTTTTTTTTTTTTTT364630.2830066619068128No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC352610.27367736899037726No Hit
TTTATTTTTTTTTTTTTTTTTTTTT337860.2622291934065649No Hit
TTTTTTTTTTTTTTTTTTTTTAATT333670.258977135393265No Hit
TTTTTTTATTTTTTTTTTTTTTTTT330800.2567495920762791No Hit
TTTTTTTTTTATTTTTTTTTTTTTT300100.2329218639120054No Hit
TTTTTTTTTATTTTTTTTTTTTTTT289770.2249042602658507No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT288590.2239884062191457No Hit
TTTTTTTTTTTTTTTTTTTTTATTT282650.21937809008573247No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC275390.21374325925600518No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT266080.2065173260570023No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT250540.19445599395039595No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA246690.19146782608614663No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT243720.18916266801944No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA243310.18884444754558488No Hit
TTTTTTTTTTTATTTTTTTTTTTTT241080.1871136386268119No Hit
TTTTTTTTTTTTATTTTTTTTTTTT240570.18671780340323602No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT218510.16959598961483602No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA217760.16901387899193032No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT217440.16876551179282387No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG201410.1563238674125858No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC199490.1548336642179472No Hit
TTTTTTTTTTTTTATTTTTTTTTTT198650.1541817003202928No Hit
TTTTTTTTTTTTTTATTTTTTTTTT196870.15280015777526323No Hit
TTTTTTTTTTTTTTTATTTTTTTTT187920.14585363767525505No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT183910.14274128621145254No Hit
TTTTTTTTTTTTTTTTTTTTTTAAG182070.14131317481659053No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT179200.13908563149960465No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT176800.1372228775063064No Hit
TTTTTTTTTTTTTTTTATTTTTTTT173110.1343588932416103No Hit
TTTTTTTTTTTTTTTTTTTTTACCA171690.1332567637955755No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT154610.12000016454326941No Hit
TTTTTTTTTTTTGTTTTTTTTTTTT153160.11887475067231836No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT147090.11416353536426813No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT146110.11340291081700468No Hit
TTTTTTTTTTGTTTTTTTTTTTTTT144010.11177300107286868No Hit
TTTTTTGTTTTTTTTTTTTTTTTTG143640.11148582649890186No Hit
TTTTTTTTTTTTTTTTTATTTTTTT141560.10987143970471003No Hit
TTTTTTTTGTTTTTTTTTTTTTTTT140320.10890901680817258No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT134860.10467125147341902No Hit
TATTTTTTTTTTTTTTTTTTTTTTT131100.10175293688391837No Hit
TTTTTTTTTTTTTTTTTTTTTTACT130900.10159770738447686No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCTA359.832997E-519.0285111
TCGCCAA359.832997E-519.0285111
TGCCCTA250.005982379419.028511
TCGCCTA401.2698736E-519.028511
TCCCCGA759.094947E-1219.028511
GGCCGAG250.00600512119.0160162
CGCCTAA401.2770874E-519.0160142
GGCTAAA250.006023608619.0058983
GCCTAAA451.6653794E-619.0058963
CGCTAAA801.8189894E-1219.0058963
CGCAAAA759.094947E-1219.0058963
GCTAAAC502.1769483E-718.9994774
CCGACAC401.2867044E-518.9994754
CGACACC359.95305E-518.9984445
CGAAACC654.8930815E-1018.9984445
ACTTTGG359.957202E-518.99741211
CTGAAAA552.8485374E-818.9974112
GTAAAGG1650.018.99733713
GACACCC359.9586854E-518.9970426
CTGTAAA359.9586854E-518.9970426
Produced by FastQC (version 0.11.5)