FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n02_S_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n02_S_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9847128
Sequences flagged as poor quality0
Sequence length25
%GC8

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT221703022.514483410797546No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA3352143.4041803864030205No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG3137703.186411306931321No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2432312.4700704611537496No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1873911.90300156553261No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT1220061.239000853853022No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA1046991.0632440240443712No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1003641.0192210358187686No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT810370.8229506105739662No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC803570.8160450437934798No Hit
TTTTTTTTTTTTTTTTTTTTTTATT716570.7276944099843122No Hit
TTTTTTTTTTTTTTTTTTTTTTACA534010.5423002524187763No Hit
TTTTTTATTTTTTTTTTTTTTTTTT453790.4608348748995646No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG445190.45210136397130213No Hit
TTTTTTTTTTTTTTTTTTTTTTACC443690.4505780771814889No Hit
TTTTTATTTTTTTTTTTTTTTTTTT403170.40942902336600073No Hit
TTTTATTTTTTTTTTTTTTTTTTTT380760.38667111872619103No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC368510.3742309432760496No Hit
TTATTTTTTTTTTTTTTTTTTTTTT364330.36998605075510343No Hit
TTTTTTTTATTTTTTTTTTTTTTTT321960.3269582765655123No Hit
TTTATTTTTTTTTTTTTTTTTTTTT310540.3153609864724009No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC288240.29271478953051083No Hit
TTTTTTTATTTTTTTTTTTTTTTTT277050.28135107007850413No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA275790.28007150917506096No Hit
TTTTTTTTTTATTTTTTTTTTTTTT252990.25691754996989985No Hit
TTTTTTTTTATTTTTTTTTTTTTTT244260.24805202085318684No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA240350.24408131995440702No Hit
TTTTTTTTTTTTTTTTTTTTTAATT236890.24056760509257114No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC218860.2222576978790161No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT218440.2218311775778684No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA211540.21482405834472754No Hit
TTTTTTTTTTTATTTTTTTTTTTTT210020.21328046106438345No Hit
TTTTTTTTTTTTATTTTTTTTTTTT209710.21296564846115537No Hit
TTTTTTTTTTTTTATTTTTTTTTTT182240.18506918971704236No Hit
TTTTTTTTTTTTTTTTTTTTTACCA181210.18402319945470394No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG178320.18108833357299714No Hit
TTTTTTTTTTTTTTTTTTTTTATTT171240.17389841992507868No Hit
TTTTTTTTTTTTTTATTTTTTTTTT171160.1738171779629553No Hit
TTTTTTTTTTTTTTTATTTTTTTTT166720.16930824906510814No Hit
TTTTTTTTTTTTTTTTTTTTTTAAG165850.16842474272701644No Hit
TTTTTTTTTTTTTTTTATTTTTTTT153680.15606580923899843No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT149660.15198340064229895No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT147210.14949536555227066No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT141840.1440419988447393No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT138510.14066030217135392No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT135190.13728876074323396No Hit
TTTTTTTTTTTTTTTTTTTTTTACT128030.13001760513319213No Hit
TTTTTTTTTTTTTTTTTATTTTTTT125430.12737724136418252No Hit
TTTTTTTTTTTTTTTTTTTTTTATA121910.12380259503075415No Hit
TATTTTTTTTTTTTTTTTTTTTTTT120540.1224113264293914No Hit
TTTTTTTTTTTTTTTTTTTTTACCC120010.12187309843032405No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT112480.11422619874546162No Hit
TTTTTTTTTTTTTTTTTTTTTACAA111980.11371843648219054No Hit
TTTTTTTTTTTTTTTTTTATTTTTT105710.10735109770077123No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA101540.10311636042509044No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT100020.10157276314474636No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCCGA401.2702714E-519.027731
CCCGAAA502.1676169E-719.0071353
CCTAAAC1900.018.9993134
CTACACC250.006036812418.9986385
CTAAAAC1150.018.9986385
AAAGTGG250.00603911518.99738311
GTAAAGT401.2879322E-518.99728813
TAAGTCG359.958171E-518.99709510
GTCTGAA250.00603964718.99709510
GTAAAAC307.735471E-418.9968058
ATAATGG250.0060407118.9965179
AAAACTG307.7402283E-418.9950717
AAACTTG250.006044254618.99458718
AAACTGT250.006044254618.99458718
AACTTGT359.974485E-518.99304419
AACGGTT401.2904162E-518.99304219
GTGAAAA1350.018.29396212
TGTGAAA1250.018.23748811
GGTAAAG1150.018.17159512
GTAAAGG853.6379788E-1217.879813
Produced by FastQC (version 0.11.5)