FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n02_S_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n02_S_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10030662
Sequences flagged as poor quality0
Sequence length25
%GC7

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT234357523.364110962965356No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA3536153.5253406006502863No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG2967482.958408926549414No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2605592.597625161729106No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1986051.979978988425689No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT1189281.1856445765992314No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA1121991.118560270498597No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC989040.9860166756690636No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT894700.8919650567430146No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC799420.7969763112344928No Hit
TTTTTTTTTTTTTTTTTTTTTTATT763030.7606975491747204No Hit
TTTTTTTTTTTTTTTTTTTTTTACA576820.5750567609595458No Hit
TTTTTTATTTTTTTTTTTTTTTTTT477680.47621981480384845No Hit
TTTTTTTTTTTTTTTTTTTTTTACC439810.4384655768482678No Hit
TTTTTATTTTTTTTTTTTTTTTTTT428690.4273795687662489No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG425550.4242491672035205No Hit
TTTTATTTTTTTTTTTTTTTTTTTT410740.4094844388137094No Hit
TTATTTTTTTTTTTTTTTTTTTTTT394220.393014937598336No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC363860.3627477428708095No Hit
TTTTTTTTATTTTTTTTTTTTTTTT337210.3361792073145322No Hit
TTTATTTTTTTTTTTTTTTTTTTTT334860.33383639085835015No Hit
TTTTTTTATTTTTTTTTTTTTTTTT308930.30798565438652004No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC286540.2856640967465557No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA284020.28315179995099027No Hit
TTTTTTTTTTATTTTTTTTTTTTTT278920.2780673897694888No Hit
TTTTTTTTTATTTTTTTTTTTTTTT264550.2637413163757288No Hit
TTTTTTTTTTTTTTTTTTTTTAATT263560.2627543426346138No Hit
TTTTTTTTTTTTATTTTTTTTTTTT230760.23005460656534932No Hit
TTTTTTTTTTTATTTTTTTTTTTTT230520.2298153402038669No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT226820.2261266504643462No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA224610.22392340605236227No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA224570.22388352832544853No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC214090.21343556387404938No Hit
TTTTTTTTTTTTTTTTTTTTTACCA199670.19905964332164716No Hit
TTTTTTTTTTTTTATTTTTTTTTTT196910.1963080801645993No Hit
TTTTTTTTTTTTTTATTTTTTTTTT190860.19027657396889658No Hit
TTTTTTTTTTTTTTTATTTTTTTTT182640.1820817010881236No Hit
TTTTTTTTTTTTTTTTTTTTTATTT180690.1801376619010789No Hit
TTTTTTTTTTTTTTTTATTTTTTTT172170.17164370606845292No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG171180.1706567323273379No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT165350.16484455362966072No Hit
TTTTTTTTTTTTTTTTTTTTTTAAG158620.15813512607642446No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT156640.15616117859419448No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT149190.14873395195651096No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT148400.14794636684996462No Hit
TTTTTTTTTTTTTTTTTTTTTTACT143830.1433903365500702No Hit
TTTTTTTTTTTTTTTTTATTTTTTT140770.14033969044116928No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT134360.1339492847032429No Hit
TTTTTTTTTTTTTTTTTTTTTTATA125070.12468768262752748No Hit
TTTTTTTTTTTTTTTTTTTTTACAA122990.12261404082801314No Hit
TATTTTTTTTTTTTTTTTTTTTTTT122160.1217865779945531No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT119900.11953348642392694No Hit
TTTTTTTTTTTTTTTTTTATTTTTT116770.11641305429292703No Hit
TTTTTTTTTTTTTTTTTTTTTACCC115030.11467837317217947No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA106630.10630405052029467No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT102390.10207701146743856No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT102370.10205707260398167No Hit
TTTTTTTTTTTTTTTTTTTTTAACA100560.10025260546113507No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAAA250.006022094319.0066743
CCTAACC359.949021E-518.9993764
CCTCACC250.00603546718.9993744
CTAACCC359.952828E-518.998435
GGTAAAG1350.018.99757812
GCAAAAA250.006039118418.99738514
CAAAAAG250.006039292618.99729215
GTCTGGC250.0060422518.9956829
AAATTGC307.745051E-418.99331519
AAATTGA359.973414E-518.99331519
AACTTTG250.006046600618.99331519
TACCCAG359.973414E-518.99331519
GGGGACA359.973414E-518.99331519
GTAAAAG1700.018.43882413
GTAAAGG1250.018.23767313
GTGAAAA1050.018.0929312
TAAAAGG1950.018.02316314
TCCCCTA1800.017.9725361
CTAAACC2200.017.7030815
CCTAAAC2100.017.6422774
Produced by FastQC (version 0.11.5)