FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n02_S_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n02_S_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10244819
Sequences flagged as poor quality0
Sequence length25
%GC8

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT232747622.7185663309425No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA3234643.1573422624645686No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG3051822.97889108631397No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2337682.281816789540157No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1973941.926769033205955No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT1253881.22391620584024No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA997740.9738971474264211No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC961520.9385426916766417No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT855030.8345974682422402No Hit
TTTTTTTTTTTTTTTTTTTTTTATT787730.7689057268849747No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC754530.7364991026195777No Hit
TTTTTTTTTTTTTTTTTTTTTTACA513690.5014144222557763No Hit
TTTTTTATTTTTTTTTTTTTTTTTT473190.46188224506455405No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG451190.44040797597302594No Hit
TTTTTATTTTTTTTTTTTTTTTTTT433920.42355067473617636No Hit
TTTTTTTTTTTTTTTTTTTTTTACC416160.40621508296047004No Hit
TTTTATTTTTTTTTTTTTTTTTTTT407060.39733254438170157No Hit
TTATTTTTTTTTTTTTTTTTTTTTT387390.37813259560759444No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC350020.34165562124621235No Hit
TTTTTTTTATTTTTTTTTTTTTTTT335300.327287383017699No Hit
TTTATTTTTTTTTTTTTTTTTTTTT318270.3106643465345752No Hit
TTTTTTTATTTTTTTTTTTTTTTTT299100.29195244933073No Hit
TTTTTTTTTTATTTTTTTTTTTTTT273550.26701301409034167No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC270870.26439705767373733No Hit
TTTTTTTTTATTTTTTTTTTTTTTT269960.2635088038158605No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA265850.25949701990830687No Hit
TTTTTTTTTTTTTTTTTTTTTAATT259060.2528692795841488No Hit
TTTTTTTTTTTTATTTTTTTTTTTT229280.22380092805934396No Hit
TTTTTTTTTTTATTTTTTTTTTTTT228590.22312741689238239No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT222460.21714390464097022No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA214370.20924723023413103No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC204070.19919336788673378No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA202970.19811965443215737No Hit
TTTTTTTTTTTTTATTTTTTTTTTT191690.18710921100704658No Hit
TTTTTTTTTTTTTTTTTTTTTATTT190400.18585003795577063No Hit
TTTTTTTTTTTTTTATTTTTTTTTT188520.1840149640515855No Hit
TTTTTTTTTTTTTTTATTTTTTTTT181530.17719200309932268No Hit
TTTTTTTTTTTTTTTTTTTTTACCA175640.1714427556016363No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG174540.1703690421470599No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT171550.1674504937568931No Hit
TTTTTTTTTTTTTTTTATTTTTTTT168430.16440505195845823No Hit
TTTTTTTTTTTTTTTTTTTTTTAAG161780.1579139660739736No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT160140.15631315692351422No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT157330.15357030709864178No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT151970.1483383942654331No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT149190.14562482753477635No Hit
TTTTTTTTTTTTTTTTTATTTTTTT140970.13760125971966902No Hit
TTTTTTTTTTTTTTTTTTTTTTACT133620.13042690163681758No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT131780.12863087185825342No Hit
TATTTTTTTTTTTTTTTTTTTTTTT125670.12266688166965174No Hit
TTTTTTTTTTTTTTTTTTTTTTATA117440.11463355282313918No Hit
TTTTTTTTTTTTTTTTTTATTTTTT117230.11442857116362914No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT111860.10918689729901523No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT109780.10715660276672531No Hit
TTTTTTTTTTTTTTTTTTTTTACCC109480.10686377182456812No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT108560.10596575693528604No Hit
TTTTTTTTTTTTTTTTTTTTTACAA107850.10527272370551397No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT107740.10516535236005634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA451.647657E-619.0270711
CCCCGAA401.2781815E-519.0140532
CCCTCAC359.920717E-519.0064373
CCCTCAA706.366463E-1119.0064373
CCTCACC359.946779E-518.999944
CCTCAAC801.8189894E-1218.999944
TCTGAAA401.2875291E-518.99799211
CTGAAAA401.2877459E-518.99762212
GGCAAAA502.179313E-718.99743713
GTAAAGT451.6724225E-618.99743713
ACACCCG250.00603920518.99734516
GTAAGTG401.2881797E-518.996889
AAACGGT401.289536E-518.9945618
AACTGTT307.74724E-418.99252319
TATTACG250.006048065618.99252319
AACGGTT250.006048065618.99252319
TTAACGT250.006048065618.99252319
TCCCCTA2650.018.6680681
GGTAAAG1600.018.40394612
GTAAAGG1250.018.2375413
Produced by FastQC (version 0.11.5)