FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n02_S_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n02_S_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10295966
Sequences flagged as poor quality0
Sequence length25
%GC9

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT227377922.084173549135652No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA3288413.193881953378634No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG3202303.1102472560612573No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2362602.2946851223090676No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1928651.8732093715150187No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT1277491.2407675005919794No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA1009330.9803159800644253No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1002400.9735851886068778No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT828080.8042761601971102No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC779410.7570052193257049No Hit
TTTTTTTTTTTTTTTTTTTTTTATT749540.7279938570115713No Hit
TTTTTTTTTTTTTTTTTTTTTTACA509660.49500940465421117No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG463630.4503025748142525No Hit
TTTTTTATTTTTTTTTTTTTTTTTT461560.4482920786645955No Hit
TTTTTTTTTTTTTTTTTTTTTTACC426900.4146284088350719No Hit
TTTTTATTTTTTTTTTTTTTTTTTT412510.40065206120533037No Hit
TTTTATTTTTTTTTTTTTTTTTTTT387950.37679805857944754No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC364170.3537016342128558No Hit
TTATTTTTTTTTTTTTTTTTTTTTT359000.348680250109606No Hit
TTTTTTTTATTTTTTTTTTTTTTTT319960.31076248697790965No Hit
TTTATTTTTTTTTTTTTTTTTTTTT298510.28992908484740526No Hit
TTTTTTTATTTTTTTTTTTTTTTTT288180.2798960291826916No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC274990.26708518656724395No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA271030.2632390200200739No Hit
TTTTTTTTTATTTTTTTTTTTTTTT258500.2510692051624879No Hit
TTTTTTTTTTATTTTTTTTTTTTTT252100.24485317841958687No Hit
TTTTTTTTTTTTTTTTTTTTTAATT243980.2369665944895311No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT224500.21804656309082604No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA218850.21255897698185872No Hit
TTTTTTTTTTTATTTTTTTTTTTTT216620.21039308016362912No Hit
TTTTTTTTTTTTATTTTTTTTTTTT213790.2076444308382526No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC208830.20282701011250423No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA204200.19833010326568679No Hit
TTTTTTTTTTTTTATTTTTTTTTTT182490.17724417504875212No Hit
TTTTTTTTTTTTTTTTTTTTTATTT178630.1734951339194399No Hit
TTTTTTTTTTTTTTATTTTTTTTTT177260.17216451569478766No Hit
TTTTTTTTTTTTTTTTTTTTTACCA171420.16649239129189045No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG170860.1659484889518866No Hit
TTTTTTTTTTTTTTTATTTTTTTTT170450.16555027473866948No Hit
TTTTTTTTTTTTTTTTTTTTTTAAG170430.16553084965509793No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT167650.1628307630386503No Hit
TTTTTTTTTTTTTTTTATTTTTTTT160120.15551721907395577No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT159110.1545362523535917No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT154450.15001020788141686No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT152800.1484076384867627No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT141000.13694683917953887No Hit
TTTTTTTTTTTTTTTTTATTTTTTT132360.12855520307662244No Hit
TTTTTTTTTTTTTTTTTTTTTTACT130440.12669039505375213No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT124190.1206200564376378No Hit
TATTTTTTTTTTTTTTTTTTTTTTT119020.11559867233438806No Hit
TTTTTTTTTTTTTTTTTTTTTTATA118960.11554039708367335No Hit
TTTTTTTTTTTTTTTTTTTTTACCC111180.10798403957433425No Hit
TTTTTTTTTTTTTTTTTTATTTTTT110260.10709048573004223No Hit
TTTTTTTTTTTTTTTTTTTTTACAA107290.10420586081966471No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT105260.10223421483715078No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT104950.10193312604179151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA552.7939677E-819.0293941
CGCTAAA307.7088695E-419.0065523
CCCTAAC401.2825396E-519.006553
CCTAAAC1900.018.9987954
GTAAAAG1250.018.99787313
CGCAACC250.00603891418.9975035
GCAACCC250.00604111718.9963046
GCTCTGC250.00604179518.9959357
CTGTAAG250.00604179518.9959357
AAAACTG250.006041964518.99584417
ACCCGTG250.006043320518.99510618
AACTTTG307.742442E-418.99427419
AACGGTT552.8538125E-818.99427419
CTAAACC2300.018.5845155
CCCTAAA2200.018.1426183
CTCAACC1050.018.092865
TAAAAGG1000.018.0479814
TCCCCTA2450.017.864331
AAAACGG701.3187673E-917.63899617
AAACGGT651.007902E-817.53394118
Produced by FastQC (version 0.11.5)