FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n02_S_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n02_S_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10603993
Sequences flagged as poor quality0
Sequence length25
%GC8

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT232917521.965074854349677No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA3250033.0649114913599056No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG3044732.871305177210132No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2328192.195578590065082No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT2051441.9345919975616732No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT1289871.2164002748775862No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA1024690.9663246665666415No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC984850.9287539137379665No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT939090.8856003582801308No Hit
TTTTTTTTTTTTTTTTTTTTTTATT845490.7973317221163764No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC781960.7374203283612126No Hit
TTTTTTTTTTTTTTTTTTTTTTACA526890.49687886440513496No Hit
TTTTTTATTTTTTTTTTTTTTTTTT470170.44338957975547516No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG468780.442078752786804No Hit
TTTTTTTTTTTTTTTTTTTTTTACC440860.4157490484952225No Hit
TTTTTATTTTTTTTTTTTTTTTTTT415490.3918240987145125No Hit
TTTTATTTTTTTTTTTTTTTTTTTT411620.3881745301038958No Hit
TTATTTTTTTTTTTTTTTTTTTTTT389970.36775769278610426No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC372660.3514336533417176No Hit
TTTTTTTTATTTTTTTTTTTTTTTT336790.31760677322212494No Hit
TTTATTTTTTTTTTTTTTTTTTTTT315730.2977463300852802No Hit
TTTTTTTATTTTTTTTTTTTTTTTT304080.2867599026140436No Hit
TTTTTTTTTTTTTTTTTTTTTAATT294550.2777727220302767No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC285130.2688892759548219No Hit
TTTTTTTTTTATTTTTTTTTTTTTT277310.2615146954548159No Hit
TTTTTTTTTATTTTTTTTTTTTTTT277210.26142039135635037No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA274560.2589213327470133No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT241880.2281027533684717No Hit
TTTTTTTTTTTTATTTTTTTTTTTT236800.22331210516642175No Hit
TTTTTTTTTTTATTTTTTTTTTTTT235340.22193526532882474No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA215910.20361197899696842No Hit
TTTTTTTTTTTTTTTTTTTTTATTT210580.1985855705487546No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC208410.19653917161205217No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA205720.1940023913633289No Hit
TTTTTTTTTTTTTTATTTTTTTTTT204030.1924086520992611No Hit
TTTTTTTTTTTTTATTTTTTTTTTT200710.18927775603020486No Hit
TTTTTTTTTTTTTTTATTTTTTTTT194430.18335545864656833No Hit
TTTTTTTTTTTTTTTTTTTTTACCA182700.17229358789655932No Hit
TTTTTTTTTTTTTTTTATTTTTTTT179760.16952104740167218No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG174880.16491900739655335No Hit
TTTTTTTTTTTTTTTTTTTTTTAAG174810.16485299452762747No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT167690.15813854271688035No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT166430.1569503110762144No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT165080.15567720574692948No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT161590.1523859927104818No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT153980.14520945081725345No Hit
TTTTTTTTTTTTTTTTTTTTTTACT143280.13511891228143966No Hit
TTTTTTTTTTTTTTTTTATTTTTTT141130.13309137416443031No Hit
TTTTTTTTTTTTTTTTTTATTTTTT126740.11952101439523771No Hit
TTTTTTTTTTTTTTTTTTTTTTATA126010.1188325944764392No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT124120.11705024701544031No Hit
TATTTTTTTTTTTTTTTTTTTTTTT121940.11499441766889132No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT119060.11227845963308349No Hit
TTTTTTTTTTTTTTTTTTTTTACCC114620.10809135766121308No Hit
TTTTTTTTTTTTTTTTTTTTTACAA111410.10506419610046894No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT110240.10396083814842201No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT108350.10217849068742313No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT107150.10104684150583654No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT106270.1002169654393397No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCCGA307.6450186E-419.0300791
TCGGCCT250.006005947519.0155242
CCTCAAC654.8930815E-1018.9990184
CCACCGC307.7329006E-418.99776512
CTGAAAA307.7329006E-418.99776512
GTCTAAA307.735599E-418.9967786
GTAAGTG307.7378075E-418.9959729
ACCCGTG307.7414897E-418.99462918
AAATTGA250.00604568918.99382219
CCCATCA250.00604568918.99382219
TTCCCAG250.00604568918.99382219
CCTAAAC2100.018.5466614
GTAAAAG1750.018.4547113
GTAAAGG1700.018.43874513
TAAAGGG1700.018.43865814
GGTAAAG2300.018.17177412
CTAAACC2050.018.0710455
TCCCCTA2300.017.7889881
GTAAAGT701.3169483E-917.64053213
CTCAACC651.006083E-817.5363985
Produced by FastQC (version 0.11.5)