FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n02_S_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n02_S_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11139959
Sequences flagged as poor quality0
Sequence length25
%GC7

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT252106422.630819377342412No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4232073.7989996192984195No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG3282592.9466805039408137No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA3111902.7934573188285525No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT2032491.824504021962738No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA1354421.2158213508685265No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT1176801.0563773170080788No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1121961.0071491286458056No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC957940.8599133982450026No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT911950.8186295838252188No Hit
TTTTTTTTTTTTTTTTTTTTTTATT757510.679993526008489No Hit
TTTTTTTTTTTTTTTTTTTTTTACA647920.5816179395274256No Hit
TTTTTTTTTTTTTTTTTTTTTTACC562180.504651767569342No Hit
TTTTTTATTTTTTTTTTTTTTTTTT550990.49460684729629617No Hit
TTTTTATTTTTTTTTTTTTTTTTTT506460.4546336301596801No Hit
TTTTATTTTTTTTTTTTTTTTTTTT476430.4276766189175382No Hit
TTATTTTTTTTTTTTTTTTTTTTTT450570.4044628889567726No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC445330.39975910144732135No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG433640.38926534648825906No Hit
TTTTTTTTATTTTTTTTTTTTTTTT391080.35106053801454745No Hit
TTTATTTTTTTTTTTTTTTTTTTTT377010.3384303299500474No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC368130.33045902592639703No Hit
TTTTTTTATTTTTTTTTTTTTTTTT337340.30281978596151027No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA335720.30136556157881733No Hit
TTTTTTTTTTATTTTTTTTTTTTTT308450.27688611780348565No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA307670.27618593569330013No Hit
TTTTTTTTTATTTTTTTTTTTTTTT302280.27134749777804384No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA282120.2532504832378647No Hit
TTTTTTTTTTTTTTTTTTTTTAATT268600.24111399332798264No Hit
TTTTTTTTTTTATTTTTTTTTTTTT264250.23720913155964038No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC263940.23693085405431025No Hit
TTTTTTTTTTTTATTTTTTTTTTTT257480.23113190991097904No Hit
TTTTTTTTTTTTTTTTTTTTTACCA237340.21305284875824052No Hit
TTTTTTTTTTTTTTATTTTTTTTTT228740.20533289215875927No Hit
TTTTTTTTTTTTTATTTTTTTTTTT216110.1939953279899863No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG214810.1928283578063438No Hit
TTTTTTTTTTTTTTTATTTTTTTTT214050.19214612908359896No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT212610.19085348518787187No Hit
TTTTTTTTTTTTTTTTATTTTTTTT195810.17577263973772256No Hit
TTTTTTTTTTTTTTTTTTTTTATTT179610.16123039591079286No Hit
TATTTTTTTTTTTTTTTTTTTTTTT170040.15263970002043992No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT167110.15000952876038412No Hit
TTTTTTTTTTTTTTTTTTTTTTAAG164570.14772944855542106No Hit
TTTTTTTTTTTTTTTTTTTTTACCC161950.14537755480069542No Hit
TTTTTTTTTTTTTTTTTTTTTTATA161480.14495565019584006No Hit
TTTTTTTTTTTTTTTTTTTTTACAA155230.13934521662063568No Hit
TTTTTTTTTTTTTTTTTATTTTTTT152070.13650858140501235No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT142250.12769346817165125No Hit
TTTTTTTTTTTTTTTTTTATTTTTT140260.12590710612130618No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT138580.12439902157629126No Hit
TTTTTTTTTTTTTTTTTTTTTTACT136080.1221548481462095No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA130380.11703813272562313No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT129910.11661622812076777No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC125880.11299862055147598No Hit
TTTTTTTTTTTTTTTTTTTTTAACA124210.1114995127001814No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT121170.108770597809202No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA120980.10860004062851578No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA401.2703287E-519.0276761
GCCCCTA706.366463E-1119.0276761
TCGCCAA250.005983847719.0276741
TCCGCAA250.005983847719.0276741
TCGGCCT359.885484E-519.0152762
GCCTAAA307.7102875E-419.006053
GGGCAAA250.006037824318.99811612
AAAGTGT401.2875742E-518.99794415
CTCAACC654.8930815E-1018.9978585
CTCAAAC250.00603829418.9978585
TAAAGTG401.2876739E-518.99777214
TGACTGT250.006039547318.9971756
GTCTAAA250.006039547318.9971756
ACTGTGT359.961768E-518.9962398
AAACGCA250.0060412718.9962378
AACGCAC250.0060417418.9959819
GTAAGTC250.0060417418.9959819
AAATTGC359.973443E-518.99333819
AACTGTT250.006046598318.99333819
TTACAGA250.006046598318.99333819
Produced by FastQC (version 0.11.5)