FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n02_S_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n02_S_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7162386
Sequences flagged as poor quality0
Sequence length25
%GC8

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT159581922.280550084846027No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA2196563.066799248183496No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1873122.615217889680897No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1593812.2252500772787167No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1438142.007906303849025No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT810231.1312291741886016No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA706880.9869336838310585No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT666820.931002601646993No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC617020.8614726991815298No Hit
TTTTTTTTTTTTTTTTTTTTTTATT608860.8500798476932128No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC501310.6999203896578599No Hit
TTTTTTTTTTTTTTTTTTTTTTACA356800.4981580160577774No Hit
TTTTTTATTTTTTTTTTTTTTTTTT338280.47230071096419546No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG294510.411189790664731No Hit
TTTTTATTTTTTTTTTTTTTTTTTT286470.39996448111006583No Hit
TTTTTTTTTTTTTTTTTTTTTTACC285720.39891734402474255No Hit
TTTTATTTTTTTTTTTTTTTTTTTT276880.3865750882457326No Hit
TTATTTTTTTTTTTTTTTTTTTTTT256540.3581767304917663No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC235420.32868935016906375No Hit
TTTTTTTTATTTTTTTTTTTTTTTT231570.3233140464644044No Hit
TTTTTTTTTTTTTTTTTTTTTAATT214550.299551015541469No Hit
TTTATTTTTTTTTTTTTTTTTTTTT207690.2899732016677124No Hit
TTTTTTTATTTTTTTTTTTTTTTTT203490.28410923398990223No Hit
TTTTTTTTTTATTTTTTTTTTTTTT194320.27130623789335007No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC186320.2601367756499021No Hit
TTTTTTTTTATTTTTTTTTTTTTTT184990.2582798525519289No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA173490.24222375057697254No Hit
TTTTTTTTTTTTATTTTTTTTTTTT162450.2268098926810144No Hit
TTTTTTTTTTTATTTTTTTTTTTTT161710.22577671742349545No Hit
TTTTTTTTTTTTTTTTTTTTTATTT153630.21449556055761304No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT151410.21139603478505628No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA145060.20253027412931948No Hit
TTTTTTTTTTTTTTATTTTTTTTTT140910.19673611559053086No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT136690.1908442242571121No Hit
TTTTTTTTTTTTTATTTTTTTTTTT134130.18726999633920874No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA131800.18401689046080455No Hit
TTTTTTTTTTTTTTTTTTTTTACCA129690.18107094479409513No Hit
TTTTTTTTTTTTTTTATTTTTTTTT129250.18045662437070553No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT128670.17964683835805553No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC127550.17808311364397283No Hit
TTTTTTTTTTTTTTTTATTTTTTTT123130.17191198575446787No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT116680.16290660682068797No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT116250.16230624822510265No Hit
TTTTTTTTTTTTTTTTTTTTTTAAG111490.15566041819025114No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT106680.14894477901637806No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT102360.14291326940491617No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG102010.14242460543176533No Hit
TTTTTTTTTTTTTTTTTTTTTTACT97560.13621159205884742No Hit
TTTTTTTTTTTTTTTTTATTTTTTT96430.13463390551696042No Hit
TTTTTTTTTTTTTTTTTTATTTTTT86850.12125847448043153No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT86310.12050453577899878No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT84060.11736312452302905No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT82250.11483603369044898No Hit
TTTTTTTTTTTTTTTTTTTTTTATA81580.1139005912275602No Hit
TTTTTTTTTTTTTTTTTTTTTACAA79570.11109426383889391No Hit
TTTTTTTTTTTTTTTTTTTTTACCC76590.10693363915320955No Hit
TATTTTTTTTTTTTTTTTTTTTTTT74630.10419712090356482No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT73870.10313602199043728No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT73010.10193530479926663No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTCAA307.709063E-419.0063653
CCTAACC250.006034412418.999864
CTCAACC359.951905E-518.9985335
GGTAAAG759.094947E-1218.99773812
CAAAAAG359.956706E-518.9973415
GTAAGTG250.006040500518.9965449
AAAACTG359.968451E-518.99442117
AAACTGT307.742447E-418.99415818
AAACGGT250.006044886518.99415818
GTTGAAA250.006047080718.99296419
CCCATCC250.006047080718.99296419
GGGGACA307.7457196E-418.99296419
AACGTTT359.974328E-518.99296219
GTAAAAG950.017.9977313
TTTACAG2700.017.937819
GTAAAGG751.72804E-1017.73109813
AAAGGGT701.3169483E-917.64038715
AGGGTTG701.3205863E-917.63767617
CCCTAAA651.0002623E-817.5443363
GTGAAAA651.0059011E-817.53637312
Produced by FastQC (version 0.11.5)