FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86978032
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG81058409.319410676019894No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1135210.13051686430431078No Hit
CCGAGCCCACGAGACGCCTCAATATCTCGTATGCCGTCTTCTGCTTGCCG990250.11385058700799301RNA PCR Primer, Index 6 (96% over 29bp)
CCGAGCCCACGAGACGCAACCTAATCTCGTATGCCGTCTTCTGCTTGCCG915180.10521967202017171RNA PCR Primer, Index 29 (96% over 29bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT1783000.034.922431
TCTTATA1733150.034.5985532
TCCGACG56850.033.94600341
TACTCGG352850.033.1248253
AGGCTGA1598100.032.9566511
GAGCGTT98800.032.8364038
AATCGCT276050.032.13099326
CTACTCG366250.032.0554582
GCTACTC759550.031.9179941
CGGAGCG102450.031.7988976
CGGCCTA111100.031.5369710
CGCTTGA305150.031.52131829
TATACAC1566000.031.5209855
CTACTTG418750.031.3046972
TTGAACC694550.031.19754432
ACTCGGG374800.031.1003864
CTCGGGA397000.030.99015
CTTATAC1766350.030.9681743
TCGGAGC104700.030.9474415
GGCTGAG1609650.030.7748712
Produced by FastQC (version 0.11.5)