FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n01_S_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n01_S_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12293462
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG364970.2968813829660026No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGCAACGATCTCGTATGCC221010.17977848713405548No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA134200.10916371645351No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGCAACGCTCTCGTATGCC128610.10461658400213057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACG21200.038.18778641
CGACGGC21850.036.95168343
CTACTCG172950.035.007252
GAATCGC126150.034.93288825
TACTCGG174100.034.54873
GCTACTT194350.034.503721
TTCCGAC22600.034.4604940
GCTACTC360900.034.4244041
AGGCTGA708750.034.37522511
GTATGCC61650.034.36471644
AGAATCG132450.034.3341124
AATCGCT129050.034.24975226
CTACTTG192450.034.2037622
AATGGCG82500.033.92126
GCACTTT269550.033.725351
CGTGAAC81550.033.587231
ATGGCGT81950.033.4773627
TTGGGAG501350.033.3863146
GGCTGAG703100.033.29369412
TCGCTTG147100.033.27739328
Produced by FastQC (version 0.11.5)