FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n01_S_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n01_S_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11527405
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTCGTATGCC332470.2884170374858869No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG308540.2676578119706907No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC188990.16394843418792No Hit
GCCCTTGAGAAGGATTATGAGGAGGTTGGTGTGGATTCTGTTGAAGGAGA188050.16313298613174432No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTCGTATGC182010.15789329862184942No Hit
ACAAAGGTGCTGCTTTTACAGGGAAGCTTATTCTGTTTTAAACATTGAAA160280.1390425685572772No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA144890.12569177538223042No Hit
GACAGAAATCAGGTATTGGCAGTTTTTCCATTTTCATTTGTGTGTGAATT136500.11841346773189629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG108150.035.6398772
TACTCGG108400.035.476653
GCTACTC222250.035.091741
GTATGCC68300.034.49767344
GCGTTCT15400.034.4269510
TCCGACG13400.034.31330541
CTACTTG127050.033.9744872
AGGCTGA486550.033.88818411
GCGACGT5200.033.84470413
ATACCGC8650.033.8261735
GCTACTT133300.033.784551
AGAATCG94400.033.35075424
GAATCGC90500.033.329525
AATCGCT92100.033.10880326
ACTCGGG118300.032.7865834
CTCGGGA127150.032.650075
GCACTTT157100.032.6154821
TACTCAG117350.032.6022033
TACTTGG133400.032.340913
CGACGGC13750.032.31987843