FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n01_S_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n01_S_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2576710
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTTGAGAAGGATTATGAGGAGGTTGGTGTGGATTCTGTTGAAGGAGA64280.24946540355724936No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55270.21449833314575564No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA48570.18849618311723088No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC44790.1738263133996453No Hit
GACAGAAATCAGGTATTGGCAGTTTTTCCATTTTCATTTGTGTGTGAATT43670.1694796853351755No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGCC40280.15632337360432488No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC39690.15403363203464882No Hit
ACAAAGGTGCTGCTTTTACAGGGAAGCTTATTCTGTTTTAAACATTGAAA34470.13377524051988776No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA33430.12973908588859437No Hit
CTGATCTGTGCAGGGTATTAACGTGTCAGGGCTGAGTGTTCTGGGATTTC28060.10889855668662753No Hit
CCTTTGGGTCGCTTTGCTGTTCGTGATATGAGACAGACAGTTGCGGTGGG27390.10629834168377505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACGT5400.038.29805421
GCGTTCT4700.037.44416410
TCCGACG3350.037.433141
AGACACG5600.036.93026420
CGTTCGA301.3016385E-436.66621444
GTCGAAT2950.036.53979510
CTACTCG13750.035.5202522
TACTCGG13900.035.295213
TCGGAGC4900.034.5716745
TATACCG3000.034.4669134
GAATCGC10000.034.3222425
GCGACGT1800.034.22047413
ATTAACG4650.034.06475417
GTATGCC12900.033.93756544
ATACCGC3050.033.90253435
CGTTCTC5000.033.8776111
TCGTCAC2350.033.70236628
CGGAGCG5100.033.6472976
TACTTCG3750.033.44023517
TTCCGAC3500.033.3158140
Produced by FastQC (version 0.11.5)