FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n01_S_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n01_S_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19389122
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG463920.23926818346906065No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA326070.16817161705413994No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC287450.14825323188951No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC257650.13288378916796748No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA249080.12846378500274536No Hit
GCCACCCCACTCTTAATCAGTGGTGGAAGAACGGTCTCAGAACTGTTTGT215380.11108290514650432No Hit
CCTTTGGGTCGCTTTGCTGTTCGTGATATGAGACAGACAGTTGCGGTGGG212690.10969552927667378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACG48200.038.15858541
GCGTTCT44400.037.0612710
CGGAGCG46100.035.9345786
GAGCGTT45900.035.850218
TTCCGAC50800.035.2097740
CGACGGC52100.035.00623343
TCGGAGC47500.034.504665
GAATCGC144050.034.5021425
AGAATCG148750.034.21059424
AATCGCT144800.034.18633726
TACTTCG56050.033.9514117
CTACTCG194250.033.8629882
TACTCGG195200.033.562943
GCACTTT377700.033.4863851
TTCGAGT57250.033.4715420
AGGCTGA857650.033.1091711
CTACTTG219300.033.0345342
TCGCTTG165100.032.92766628
GCTACTT228350.032.8241921
CGCTTGA162250.032.7738329