FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n01_S_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n01_S_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11072457
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA271380.2450946524335114No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG267910.24196074999433279No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC239910.2166727764217102No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC236230.21334921418073696No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA158020.1427144851409222No Hit
CCTTTGGGTCGCTTTGCTGTTCGTGATATGAGACAGACAGTTGCGGTGGG156070.1409533584099717No Hit
AATACCTGCCACCCCACTCTTAATCAGTGGTGGAAGAACGGTCTCAGAAC139730.1261960195465198No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTACCAATCTCGTATGCC123660.11168252899966107No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA119630.10804286708903002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTCT35250.038.63047410
CGGAGCG36150.037.7940146
TCGGAGC37200.036.8453645
CGTTCTC37450.036.77198811
GACACGT29500.036.39431821
GAGCGTT37550.036.3821228
ATACCGC10900.035.7256935
CGGCCTA30950.035.610310
TATACCG11100.035.08166534
GCGACGT12650.034.9542613
CTACTCG55700.034.4820332
GAATCGC49900.034.39074725
AGACACG31700.034.21461520
TCCGACG18350.034.04868741
TTCGGAG41000.033.9133724
GTCGAAT13300.033.9078910
ACGTGCC31050.033.7987324
CCTTCGG41350.033.7856332
TACTCGG56700.033.6410753
GTCCTAC40800.033.48494319