Basic Statistics
Measure | Value |
---|---|
Filename | HFJM7BGXC_n01_S_4.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 10041854 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA | 26437 | 0.2632681176205111 | No Hit |
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC | 25513 | 0.2540666295287703 | No Hit |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 20332 | 0.20247257130008067 | No Hit |
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC | 20066 | 0.1998236580615492 | No Hit |
CCTTTGGGTCGCTTTGCTGTTCGTGATATGAGACAGACAGTTGCGGTGGG | 16474 | 0.164053371020929 | No Hit |
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA | 16270 | 0.16202187365002518 | No Hit |
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA | 10998 | 0.1095216082607853 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GAGCGTT | 3655 | 0.0 | 39.12267 | 8 |
GCGTTCT | 3770 | 0.0 | 37.69567 | 10 |
TCGGAGC | 3830 | 0.0 | 37.16628 | 5 |
CGGAGCG | 3815 | 0.0 | 37.139587 | 6 |
CGTTCTC | 3955 | 0.0 | 36.210526 | 11 |
GACACGT | 2680 | 0.0 | 36.03833 | 21 |
AGACACG | 2785 | 0.0 | 35.07407 | 20 |
TCCGACG | 1800 | 0.0 | 34.711224 | 41 |
CGGCCTA | 2875 | 0.0 | 34.203457 | 10 |
TTCGGAG | 4195 | 0.0 | 33.93233 | 4 |
CTACTCG | 3580 | 0.0 | 33.80017 | 2 |
TATACCG | 985 | 0.0 | 33.725494 | 34 |
GTCCTAC | 4190 | 0.0 | 33.603764 | 19 |
ACGTGCC | 2840 | 0.0 | 33.5437 | 24 |
ATACCGC | 995 | 0.0 | 33.38721 | 35 |
CCTTCGG | 4310 | 0.0 | 33.077827 | 2 |
TGGGTCG | 2945 | 0.0 | 33.020264 | 5 |
CGTGATA | 2870 | 0.0 | 32.885616 | 22 |
GGAGCGT | 4335 | 0.0 | 32.884285 | 7 |
GAATCGC | 3200 | 0.0 | 32.795162 | 25 |