FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n01_S_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n01_S_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10041854
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA264370.2632681176205111No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC255130.2540666295287703No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG203320.20247257130008067No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC200660.1998236580615492No Hit
CCTTTGGGTCGCTTTGCTGTTCGTGATATGAGACAGACAGTTGCGGTGGG164740.164053371020929No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA162700.16202187365002518No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA109980.1095216082607853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTT36550.039.122678
GCGTTCT37700.037.6956710
TCGGAGC38300.037.166285
CGGAGCG38150.037.1395876
CGTTCTC39550.036.21052611
GACACGT26800.036.0383321
AGACACG27850.035.0740720
TCCGACG18000.034.71122441
CGGCCTA28750.034.20345710
TTCGGAG41950.033.932334
CTACTCG35800.033.800172
TATACCG9850.033.72549434
GTCCTAC41900.033.60376419
ACGTGCC28400.033.543724
ATACCGC9950.033.3872135
CCTTCGG43100.033.0778272
TGGGTCG29450.033.0202645
CGTGATA28700.032.88561622
GGAGCGT43350.032.8842857
GAATCGC32000.032.79516225