FastQCFastQC Report
Wed 18 Sep 2019
HFJM7BGXC_n01_S_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJM7BGXC_n01_S_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6271747
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG200100.31904985963241184No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC116210.185291275301762No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA111950.17849890947450528No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCAATATCTCGTATGCC107300.1710847073391194No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC90910.14495163787697432No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA89920.14337313032556956No Hit
CCTTTGGGTCGCTTTGCTGTTCGTGATATGAGACAGACAGTTGCGGTGGG74320.11849967800040404No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA63980.10201304357462122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTCT16550.037.2184210
GAGCGTT16950.036.9893348
CGGAGCG16800.036.6666226
TCCGACG10150.036.41403641
GACACGT15550.035.93736621
CGGCCTA16000.035.33548410
TCGGAGC17550.035.0996675
CGACGGC10500.034.99043343
CGTTCTC18050.034.49055511
ATACCGC5200.034.27000835
TTCGGAG18300.033.661164
CTACTCG42650.033.5281452
TACTCGG42900.033.4355853
AATCGCT36000.033.4290726
TATACCG5350.033.3091734
ACACGTG16900.033.1960222
TTCGAGT11600.033.1901420
AGACACG17200.033.1283920
TTCCGAC11000.033.00153740
GAATCGC36150.032.92572425